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BMRB: 25417

NMR resonance assignments of CadC 1-159 from E.coli

Authors

Schlundt, A., Sattler, M., Heydenreich, T.

Assembly

CadC1-159

Entity

1. CadC1-159 (polymer, Thiol state: not present), 162 monomers, 18160.39 Da Detail

GAMAQQPVVR VGEWLVTPSI NQISRNGRQL TLEPRLIDLL VFFAQHSGEV LSRDELIDNV WKRSIVTNHV VTQSISELRK SLKDNDEDSP VYIATVPKRG YKLMVPVIWY SEEEGEEIML SSPPPIPEAV PATDSPSHSL NIQNTATPPE QSPVKSKRFT TF

Formula weight

18160.39 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 68.5 %, Completeness (bb): 84.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	68.5 % (1301 of 1900)	64.4 % (639 of 992)	68.4 % (508 of 743)	93.3 % (154 of 165)
Backbone	84.0 % (791 of 942)	80.6 % (254 of 315)	82.7 % (397 of 480)	95.2 % (140 of 147)
Sidechain	58.6 % (653 of 1114)	56.9 % (385 of 677)	60.6 % (254 of 419)	77.8 % (14 of 18)
Aromatic	64.3 % (72 of 112)	76.8 % (43 of 56)	49.1 % (26 of 53)	100.0 % (3 of 3)
Methyl	69.6 % (135 of 194)	76.3 % (74 of 97)	62.9 % (61 of 97)

1. CadC

GAMAQQPVVR VGEWLVTPSI NQISRNGRQL TLEPRLIDLL VFFAQHSGEV LSRDELIDNV WKRSIVTNHV VTQSISELRK SLKDNDEDSP VYIATVPKRG YKLMVPVIWY SEEEGEEIML SSPPPIPEAV PATDSPSHSL NIQNTATPPE QSPVKSKRFT TF

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	CadC	[U-100% 13C; U-100% 15N]	1 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %
4	BisTrisnatural	abundance"	20 mM
5	NaCl	natural abundance	150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.24 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.24 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 1 Detail

Release date

2015-05-17

Citation

Structural and Functional Analysis of the Signal-Transducing Linker in the pH-Responsive One-Component System CadC of Escherichia coli
Schlundt, A., Heydenreich, T., Buchner, S., Janowski, R., Niessing, D., Sattler, M., Jung, K.
J. Mol. Biol. (2015), 427, 2548-2561, PubMed 25979249 , DOI 10.1016/j.jmb.2015.05.001 , Abstract

Related entities 1. CadC1-159, : 2 : 1 entities Detail

Interaction partners 1. CadC1-159, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC