Solution structure of hnRNP C RRM in complex with the 5'-AUUUUUC-3' RNA
ASNVTNKTDP RSMNSRVFIG NLNTLVVKKS DVEAIFSKYG KIVGCSVHKG FAFVQYVNER NARAAVAGED GRMIAGQVLD INLAAEPKVN RGKAGVKRSA AEMYG
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 96.4 % (1249 of 1295) | 98.8 % (666 of 674) | 93.4 % (469 of 502) | 95.8 % (114 of 119) |
Backbone | 99.1 % (697 of 703) | 100.0 % (260 of 260) | 98.2 % (334 of 340) | 100.0 % (103 of 103) |
Sidechain | 94.2 % (647 of 687) | 98.1 % (406 of 414) | 89.5 % (230 of 257) | 68.8 % (11 of 16) |
Aromatic | 66.0 % (70 of 106) | 92.5 % (49 of 53) | 43.8 % (21 of 48) | 0.0 % (0 of 5) |
Methyl | 100.0 % (120 of 120) | 100.0 % (60 of 60) | 100.0 % (60 of 60) |
1. hnRNP C
ASNVTNKTDP RSMNSRVFIG NLNTLVVKKS DVEAIFSKYG KIVGCSVHKG FAFVQYVNER NARAAVAGED GRMIAGQVLD INLAAEPKVN RGKAGVKRSA AEMYG2. RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3')
AUUUUUCSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | hnRNP C | [U-100% 15N] | 1.0 mM | |
2 | RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3') | natural abundance | 1.0 mM | |
3 | sodium phosphate | natural abundance | 20 mM | |
4 | sodium chloride | natural abundance | 10 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | hnRNP C | [U-100% 13C; U-100% 15N] | 1.0 mM | |
8 | RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3') | natural abundance | 1.0 mM | |
9 | sodium phosphate | natural abundance | 20 mM | |
10 | sodium chloride | natural abundance | 10 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | hnRNP C | [U-100% 13C; U-100% 15N] | 1.0 mM | |
14 | RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3') | natural abundance | 1.0 mM | |
15 | sodium phosphate | natural abundance | 20 mM | |
16 | sodium chloride | natural abundance | 10 mM | |
17 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
18 | hnRNP C | [U-100% 15N] | 0.5 ~ 1.0 mM | |
19 | RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3') | [100% 13C5]-Ura | 0.5 ~ 1.0 mM | |
20 | sodium phosphate | natural abundance | 20 mM | |
21 | sodium chloride | natural abundance | 10 mM | |
22 | D2O | natural abundance | 100 % |