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BMRB: 25449

2MYF

Solution structure of RNA recognition motif of a cyclophilin33 - like protein from Plasmodium falciparum

Authors

Ganguly, A., Bhavesh, N.

Assembly

RNA recognition motif of a cyclophilin33 - like protein from Plasmodium falciparum

Entity

1. RNA recognition motif of a cyclophilin33 - like protein from Plasmodium falciparum (polymer, Thiol state: not present), 89 monomers, 10137.15 Da Detail

GSHMSDNNAT DILFVGGIDE TIDEKSLYDI FSSFGDIRNI EVPLNMTTKK NRGFAFVEYV EVDDAKHALY NMNNFELNGK RIHVNYSKT

Formula weight

10137.15 Da

Source organism

Plasmodium falciparum 3D7

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.5 %, Completeness (bb): 97.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.5 % (928 of 1037)	86.7 % (464 of 535)	90.8 % (367 of 404)	99.0 % (97 of 98)
Backbone	97.2 % (517 of 532)	96.2 % (176 of 183)	97.7 % (255 of 261)	97.7 % (86 of 88)
Sidechain	81.6 % (480 of 588)	79.5 % (280 of 352)	84.1 % (190 of 226)	100.0 % (10 of 10)
Aromatic	49.0 % (51 of 104)	50.0 % (26 of 52)	48.1 % (25 of 52)
Methyl	96.7 % (87 of 90)	95.6 % (43 of 45)	97.8 % (44 of 45)

1. RRM

GSHMSDNNAT DILFVGGIDE TIDEKSLYDI FSSFGDIRNI EVPLNMTTKK NRGFAFVEYV EVDDAKHALY NMNNFELNGK RIHVNYSKT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	RRM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DSS	natural abundance	50 uM
5	D2O	natural abundance	10 %
6	H2O	natural abundance	90 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	RRM	[U-100% 13C; U-100% 15N]	0.5 ~ 1.0 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	50 mM
10	DSS	natural abundance	50 uM
11	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MYF, Strand ID: A Detail

Release date

2016-01-24

Citation

Solution structure of RNA recognition motif of a cyclophilin33 - like protein from Plasmodium falciparum
Ganguly, A., Bhavesh, N.
Not known

Related entities 1. RNA recognition motif of a cyclophilin33 - like protein from Plasmodium falciparum, : 1 : 1 : 1 : 419 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC