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Solution structure of RNA recognition motif of a cyclophilin33 - like protein from Plasmodium falciparum
Authors
Ganguly, A., Bhavesh, N.
Assembly
RNA recognition motif of a cyclophilin33 - like protein from Plasmodium falciparum
Entity
1. RNA recognition motif of a cyclophilin33 - like protein from Plasmodium falciparum (polymer, Thiol state: not present), 89 monomers, 10137.15 Da Detail

GSHMSDNNAT DILFVGGIDE TIDEKSLYDI FSSFGDIRNI EVPLNMTTKK NRGFAFVEYV EVDDAKHALY NMNNFELNGK RIHVNYSKT


Formula weight
10137.15 Da
Source organism
Plasmodium falciparum 3D7
Exptl. method
solution NMR
Refine. method
DGSA-distance geometry simulated annealing, molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 89.5 %, Completeness (bb): 97.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All89.5 % (928 of 1037)86.7 % (464 of 535)90.8 % (367 of 404)99.0 % (97 of 98)
Backbone97.2 % (517 of 532)96.2 % (176 of 183)97.7 % (255 of 261)97.7 % (86 of 88)
Sidechain81.6 % (480 of 588)79.5 % (280 of 352)84.1 % (190 of 226)100.0 % (10 of 10)
Aromatic49.0 % (51 of 104)50.0 % (26 of 52)48.1 % (25 of 52)
Methyl96.7 % (87 of 90)95.6 % (43 of 45)97.8 % (44 of 45)

1. RRM

GSHMSDNNAT DILFVGGIDE TIDEKSLYDI FSSFGDIRNI EVPLNMTTKK NRGFAFVEYV EVDDAKHALY NMNNFELNGK RIHVNYSKT

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8


#NameIsotope labelingTypeConcentration
1RRM[U-100% 13C; U-100% 15N]0.5 ~ 1.0 mM
2sodium phosphatenatural abundance20 mM
3sodium chloridenatural abundance50 mM
4DSSnatural abundance50 uM
5D2Onatural abundance10 %
6H2Onatural abundance90 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8


#NameIsotope labelingTypeConcentration
7RRM[U-100% 13C; U-100% 15N]0.5 ~ 1.0 mM
8sodium phosphatenatural abundance20 mM
9sodium chloridenatural abundance50 mM
10DSSnatural abundance50 uM
11D2Onatural abundance100 %

LACS Plot; CA
Referencing offset: -0.13 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.13 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.05 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.31 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2MYF, Strand ID: A Detail


Release date
2016-01-24
Citation
Solution structure of RNA recognition motif of a cyclophilin33 - like protein from Plasmodium falciparum
Ganguly, A., Bhavesh, N.
Not known
Related entities 1. RNA recognition motif of a cyclophilin33 - like protein from Plasmodium falciparum, : 1 : 1 : 1 : 419 entities Detail
Experiments performed 12 experiments Detail
NMR combined restraints 4 contents Detail
Keywords PfCyp33, RNA recognition motif