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BMRB: 25483

2MZC

Metal Binding of Glutaredoxins

Authors

Bilinovich, S.M., Caporoso, J.A., Taraboletti, A., Duangjumpa, N., Panzner, M.J., Prokop, J.W., Shriver, L.P., Leeper, T.C.

Assembly

Metal Binding of Glutaredoxins

Entity

1. Glutaredoxin (polymer, Thiol state: free and other bound), 92 monomers, 9928.192 × 2 Da Detail

GPGSMVDVII YTRPGCPYCA RAKALLARKG AEFNEIDASA TPELRAEMQE RSGRNTFPQI FIGSVHVGGS DDLYALEDEG KLDSLLKTGK LI

2. SILVER ION (non-polymer), 107.868 Da

Total weight

19964.252 Da

Max. entity weight

9928.192 Da

Source organism

Brucella melitensis

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 90.4 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.4 % (928 of 1027)	89.1 % (476 of 534)	90.3 % (363 of 402)	97.8 % (89 of 91)
Backbone	97.4 % (528 of 542)	97.4 % (184 of 189)	97.0 % (258 of 266)	98.9 % (86 of 87)
Sidechain	83.1 % (471 of 567)	83.2 % (287 of 345)	83.0 % (181 of 218)	75.0 % (3 of 4)
Aromatic	75.9 % (44 of 58)	96.6 % (28 of 29)	55.2 % (16 of 29)
Methyl	91.2 % (93 of 102)	92.2 % (47 of 51)	90.2 % (46 of 51)

1. Glutaredoxin

GPGSMVDVII YTRPGCPYCA RAKALLARKG AEFNEIDASA TPELRAEMQE RSGRNTFPQI FIGSVHVGGS DDLYALEDEG KLDSLLKTGK LI

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Glutaredoxin	[U-99% 13C; U-99% 15N]	1.0 mM
2	SILVER ION	natural abundance	0.5 mM
3	MES	natural abundance	50 mM
4	TCEP	natural abundance	0.01 mM
5	D2O	[U-99% 2H]	5 %
6	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514
¹H	water	protons	4.71 ppm	na	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013

Referencing offset: -0.43 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514
¹H	water	protons	4.71 ppm	na	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013

Referencing offset: -0.43 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514
¹H	water	protons	4.71 ppm	na	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514
¹H	water	protons	4.71 ppm	na	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2MZC, Strand ID: A, B Detail

Release date

2016-04-11

Citation

Metal Binding of Glutaredoxins
Bilinovich, S.M., Caporoso, J.A., Taraboletti, A., Duangjumpa, N., Panzner, M.J., Prokop, J.W., Shriver, L.P., Leeper, T.C.
Not known

Related entities 1. Glutaredoxin, : 1 : 1 : 252 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC