Characterization of the p300 Taz2-p53 TAD2 Complex and Comparison with the p300 Taz2-p53 TAD1 Complex
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 68.0 % (905 of 1331) | 68.2 % (481 of 705) | 69.5 % (349 of 502) | 60.5 % (75 of 124) |
Backbone | 71.2 % (480 of 674) | 70.9 % (161 of 227) | 72.1 % (245 of 340) | 69.2 % (74 of 107) |
Sidechain | 66.2 % (508 of 767) | 66.9 % (320 of 478) | 68.8 % (187 of 272) | 5.9 % (1 of 17) |
Aromatic | 0.0 % (0 of 56) | 0.0 % (0 of 28) | 0.0 % (0 of 27) | 0.0 % (0 of 1) |
Methyl | 76.9 % (80 of 104) | 78.8 % (41 of 52) | 75.0 % (39 of 52) |
1. Taz2 domain of Histone Acetyltransferase p300
ATQSPGDSRR LSIQRAIQSL VHAAQCRNAN CSLPSCQKMK RVVQHTKGCK RKTNGGCPIC KQLIALAAYH AKHCQENKCP VPFCLNIKQK2. TAD2 sub-domain of Cellular Tumor Antigen p53
LPSQAMDDLM LSPDDIEQWF TEDPGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Taz2 domain of Histone Acetyltransferase p300 | natural abundance | 1.1 mM | |
2 | TAD2 sub-domain of Cellular Tumor Antigen p53 | [U-100% 13C; U-100% 15N] | 1.0 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | Taz2 domain of Histone Acetyltransferase p300 | [U-100% 15N] | 1.0 mM | |
4 | TAD2 sub-domain of Cellular Tumor Antigen p53 | natural abundance | 1.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | Taz2 domain of Histone Acetyltransferase p300 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
6 | TAD2 sub-domain of Cellular Tumor Antigen p53 | natural abundance | 1.1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Taz2 domain of Histone Acetyltransferase p300 | natural abundance | 1.0 mM | |
8 | TAD2 sub-domain of Cellular Tumor Antigen p53 | natural abundance | 1.1 mM |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 46.5 % (1237 of 2662) | 46.7 % (659 of 1410) | 46.6 % (468 of 1004) | 44.4 % (110 of 248) |
Backbone | 49.4 % (666 of 1348) | 49.8 % (226 of 454) | 49.3 % (335 of 680) | 49.1 % (105 of 214) |
Sidechain | 44.6 % (684 of 1534) | 45.3 % (433 of 956) | 45.2 % (246 of 544) | 14.7 % (5 of 34) |
Aromatic | 6.3 % (7 of 112) | 10.7 % (6 of 56) | 0.0 % (0 of 54) | 50.0 % (1 of 2) |
Methyl | 50.5 % (105 of 208) | 51.9 % (54 of 104) | 49.0 % (51 of 104) |
1. Taz2 domain of Histone Acetyltransferase p300
ATQSPGDSRR LSIQRAIQSL VHAAQCRNAN CSLPSCQKMK RVVQHTKGCK RKTNGGCPIC KQLIALAAYH AKHCQENKCP VPFCLNIKQK2. TAD2 sub-domain of Cellular Tumor Antigen p53
LPSQAMDDLM LSPDDIEQWF TEDPGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Taz2 domain of Histone Acetyltransferase p300 | natural abundance | 1.1 mM | |
2 | TAD2 sub-domain of Cellular Tumor Antigen p53 | [U-100% 13C; U-100% 15N] | 1.0 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | Taz2 domain of Histone Acetyltransferase p300 | [U-100% 15N] | 1.0 mM | |
4 | TAD2 sub-domain of Cellular Tumor Antigen p53 | natural abundance | 1.1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | Taz2 domain of Histone Acetyltransferase p300 | [U-100% 13C; U-100% 15N] | 1.0 mM | |
6 | TAD2 sub-domain of Cellular Tumor Antigen p53 | natural abundance | 1.1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Taz2 domain of Histone Acetyltransferase p300 | natural abundance | 1.0 mM | |
8 | TAD2 sub-domain of Cellular Tumor Antigen p53 | natural abundance | 1.1 mM |