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NMR structure of the RRM3 domain of Gbp2
Authors
Martinez-Lumbreras, S., Seraphin, B., Perez-Canadillas, J.
Assembly
RRM3 domain of Gbp2
Entity
1. RRM3 domain of Gbp2 (polymer, Thiol state: all free), 101 monomers, 11085.12 Da Detail

GSHIDETAAK FTEGVNPGGD RNCFIYCSNL PFSTARSDLF DLFGPIGKIN NAELKPQENG QPTGVAVVEY ENLVDADFCI QKLNNYNYGG CSLQISYARR D


Formula weight
11085.12 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 91.2 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All91.2 % (1038 of 1138)94.7 % (559 of 590)84.7 % (371 of 438)98.2 % (108 of 110)
Backbone96.3 % (574 of 596)97.6 % (202 of 207)94.9 % (278 of 293)97.9 % (94 of 96)
Sidechain87.7 % (555 of 633)93.2 % (357 of 383)78.0 % (184 of 236)100.0 % (14 of 14)
Aromatic46.2 % (48 of 104)92.3 % (48 of 52) 0.0 % (0 of 52)
Methyl100.0 % (94 of 94)100.0 % (47 of 47)100.0 % (47 of 47)

1. RRM3

GSHIDETAAK FTEGVNPGGD RNCFIYCSNL PFSTARSDLF DLFGPIGKIN NAELKPQENG QPTGVAVVEY ENLVDADFCI QKLNNYNYGG CSLQISYARR D

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1RRM3[U-100% 13C; U-100% 15N]0.8 mM
2potassium phosphatenatural abundance25 mM
3sodium chloridenatural abundance25 mM
4DTTnatural abundance0.1 mM
5D2Onatural abundance10 %
6H2Onatural abundance90 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
7RRM3[U-100% 13C; U-100% 15N]0.8 mM
8potassium phosphatenatural abundance25 mM
9sodium chloridenatural abundance25 mM
10DTTnatural abundance0.1 mM
11D2Onatural abundance100 %

LACS Plot; CA
Referencing offset: -0.06 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.06 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.01 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.27 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2MZQ, Strand ID: A Detail


Release date
2015-11-29
Citation
Gbp2 interacts with THO/TREX through a novel type of RRM domain
Martinez-Lumbreras, S., Taverniti, V., Zorrilla, S., Seraphin, B., Perez-Canadillas, J.
Nucleic Acids Res. (2015), 44, 437-448, PubMed 26602689 , DOI 10.1093/nar/gkv1303 ,
Related entities 1. RRM3 domain of Gbp2, : 1 : 2 : 91 entities Detail
Interaction partners 1. RRM3 domain of Gbp2, : 64 interactors Detail
Experiments performed 11 experiments Detail
NMR combined restraints 3 contents Detail
Keywords Gbp2, RNA binding domain, RRM, THO/TREX