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NMR structure of the RRM3 domain of Hrb1
Authors
Martinez-Lumbreras, S., Seraphin, B., Perez-Canadillas, J.
Assembly
RRM3 domain of Hrb1
Entity
1. RRM3 domain of Hrb1 (polymer, Thiol state: all free), 100 monomers, 11095.10 Da Detail

GSVNEEARKF TENVVGGGER NRLIYCSNLP FSTAKSDLYD LFETIGKVNN AELRYDSKGA PTGIAVVEYD NVDDADVCIE RLNNYNYGGC DLDISYAKRL


Formula weight
11095.1 Da
Source organism
Saccharomyces cerevisiae
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 86.2 %, Completeness (bb): 84.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All86.2 % (962 of 1116)95.8 % (552 of 576)70.4 % (304 of 432)98.1 % (106 of 108)
Backbone84.6 % (504 of 596)97.6 % (202 of 207)70.8 % (206 of 291)98.0 % (96 of 98)
Sidechain89.9 % (549 of 611)94.9 % (350 of 369)81.5 % (189 of 232)100.0 % (10 of 10)
Aromatic51.2 % (44 of 86)100.0 % (43 of 43) 2.3 % (1 of 43)
Methyl100.0 % (106 of 106)100.0 % (53 of 53)100.0 % (53 of 53)

1. RRM3

GSVNEEARKF TENVVGGGER NRLIYCSNLP FSTAKSDLYD LFETIGKVNN AELRYDSKGA PTGIAVVEYD NVDDADVCIE RLNNYNYGGC DLDISYAKRL

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1RRM3[U-100% 13C; U-100% 15N]0.8 mM
2potassium phosphatenatural abundance25 mM
3sodium chloridenatural abundance25 mM
4DTTnatural abundance0.1 mM
5D2Onatural abundance10 %
6H2Onatural abundance90 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
7RRM3[U-100% 13C; U-100% 15N]0.8 mM
8potassium phosphatenatural abundance25 mM
9sodium chloridenatural abundance25 mM
10DTTnatural abundance0.1 mM
11D2Onatural abundance100 %

LACS Plot; CA
Referencing offset: -0.16 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.16 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.25 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2MZT, Strand ID: A Detail


Release date
2015-11-29
Citation
Gbp2 interacts with THO/TREX through a novel type of RRM domain
Martinez-Lumbreras, S., Taverniti, V., Zorrilla, S., Seraphin, B., Perez-Canadillas, J.
Nucleic Acids Res. (2015), 44, 437-448, PubMed 26602689 , DOI 10.1093/nar/gkv1303 ,
Related entities 1. RRM3 domain of Hrb1, : 1 : 2 : 88 entities Detail
Interaction partners 1. RRM3 domain of Hrb1, : 42 interactors Detail
Experiments performed 11 experiments Detail
NMR combined restraints 3 contents Detail
Keywords Hrb1, RNA binding domain, RRM, THO/TREX