NMR structure of Neuromedin C in 10% TFE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.3 % (100 of 112) | 98.2 % (55 of 56) | 77.3 % (34 of 44) | 91.7 % (11 of 12) |
Backbone | 80.0 % (48 of 60) | 95.5 % (21 of 22) | 64.3 % (18 of 28) | 90.0 % (9 of 10) |
Sidechain | 100.0 % (60 of 60) | 100.0 % (34 of 34) | 100.0 % (24 of 24) | 100.0 % (2 of 2) |
Aromatic | 100.0 % (20 of 20) | 100.0 % (10 of 10) | 100.0 % (9 of 9) | 100.0 % (1 of 1) |
Methyl | 100.0 % (10 of 10) | 100.0 % (5 of 5) | 100.0 % (5 of 5) |
1. Neuromedin C
GNHWAVGHLM XSolvent system trifluoroethanol/water, Pressure 1 atm, Temperature 273 K, pH 4.0, Details The sample was prepared in 10mM acetate buffer at pH 4.0 in presence of 10% TFE
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Neuromedin C | natural abundance | 5.0 (±0.1) mM | |
2 | sodium acetate | natural abundance | 10 (±0.1) mM | |
3 | DSS | natural abundance | 1.6 (±0.1) mM | |
4 | TFE | [U-99% 2H] | 10 (±0.5) % | |
5 | D2O | [U-100% 2H] | 10 (±1.0) % | |
6 | H2O | natural abundance | 80 % |