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Solution structure of human Myosin VI isoform3 (998-1071)
Authors
He, F., Walters, K.
Assembly
human Myosin VI isoform3 (998-1071)
Entity
1. human Myosin VI isoform3 (998-1071) (polymer, Thiol state: not present), 74 monomers, 8388.269 Da Detail

QQQAVLEQER RDRELALRIA QSEAELISDE AQADLALRRS LDSYPVSKND GTRPKMTPEQ MAKEMSEFLS RGPA


Formula weight
8388.269 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 97.2 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All97.2 % (827 of 851)99.1 % (449 of 453)93.8 % (300 of 320)100.0 % (78 of 78)
Backbone95.4 % (416 of 436)100.0 % (146 of 146)90.9 % (200 of 220)100.0 % (70 of 70)
Sidechain99.2 % (483 of 487)98.7 % (303 of 307)100.0 % (172 of 172)100.0 % (8 of 8)
Aromatic100.0 % (18 of 18)100.0 % (9 of 9)100.0 % (9 of 9)
Methyl100.0 % (70 of 70)100.0 % (35 of 35)100.0 % (35 of 35)

1. entity

QQQAVLEQER RDRELALRIA QSEAELISDE AQADLALRRS LDSYPVSKND GTRPKMTPEQ MAKEMSEFLS RGPA

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5


#NameIsotope labelingTypeConcentration
1entity[U-13C; U-15N]0.7 mM
2sodium phosphate buffernatural abundance20 mM
3NaClnatural abundance50 mM
4DTTnatural abundance2 mM
5NaN3natural abundance0.1 %
6H2Onatural abundance90 %
7D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: -0.02 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.02 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.05 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 2.87 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2N11, Strand ID: A Detail


Release date
2016-03-06
Citation
Myosin VI Contains a Compact Structural Motif that Binds to Ubiquitin Chains
He, F., Wollscheid, H., Nowicka, U., Biancospino, M., Valentini, E., Ehlinger, A., Acconcia, F., Magistrati, E., Polo, S., Walters, K.J.
Cell Rep. (2016), 14, 2683-2694, PubMed 26971995 , DOI 10.1016/j.celrep.2016.01.079 ,
Related entities 1. human Myosin VI isoform3 (998-1071), : 1 : 2 : 8 entities Detail
Interaction partners 1. human Myosin VI isoform3 (998-1071), : 75 interactors Detail
Experiments performed 2 experiments Detail
NMR combined restraints 5 contents Detail
Keywords MIU, Myosin VI