Backbone amide chemical shift assignments of the CN-bound yeast cytochrome c peroxidase covalently cross-linked to yeast iso-1 cytochrome c
TTPLVHVASV EKGRSYEDFQ KVYNAIALKL REDDEYDNYI GYGPVLVRLA WHTSGTWDKH DNTGGSYGGT YRFKKEFNDP SNAGLQNGFK FLEPIHKEFP WISSGDLFSL GGVTAVQEMQ GPKIPWRAGR VDTPEDTTPD NGRLPDADKD ADYVRTFFQR LNMNDREVVA LMGAHALGKT HLKNSGYEGP WGAANNCFTN EFYLNLLNED WKLEKNDANN EQWDSKSGYM MLPTDYSLIQ DPKYLSIVKE YANDQDKFFK DFSKAFEKLL ENGITFPKDA PSPFIFKTLE EQGL
AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMCFGGL KKEKDRNDLI TYLKKATE
TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKACE
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS197:SG | 2:CYS86:SG |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 15.4 % (570 of 3700) | 10.5 % (331 of 3158) | 44.1 % (239 of 542) |
Backbone | 32.6 % (499 of 1530) | 25.0 % (261 of 1044) | 49.0 % (238 of 486) |
Sidechain | 3.3 % (71 of 2170) | 3.3 % (70 of 2114) | 1.8 % (1 of 56) |
Aromatic | 0.0 % (0 of 317) | 0.0 % (0 of 308) | 0.0 % (0 of 9) |
Methyl | 5.0 % (11 of 218) | 5.0 % (11 of 218) |
1. CcP
TTPLVHVASV EKGRSYEDFQ KVYNAIALKL REDDEYDNYI GYGPVLVRLA WHTSGTWDKH DNTGGSYGGT YRFKKEFNDP SNAGLQNGFK FLEPIHKEFP WISSGDLFSL GGVTAVQEMQ GPKIPWRAGR VDTPEDTTPD NGRLPDADKD ADYVRTFFQR LNMNDREVVA LMGAHALGKT HLKNSGYEGP WGAANNCFTN EFYLNLLNED WKLEKNDANN EQWDSKSGYM MLPTDYSLIQ DPKYLSIVKE YANDQDKFFK DFSKAFEKLL ENGITFPKDA PSPFIFKTLE EQGL2. Cc
AEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMCFGGL KKEKDRNDLI TYLKKATE3. Cc1
TEFKAGSAKK GATLFKTRCL QCHTVEKGGP HKVGPNLHGI FGRHSGQAEG YSYTDANIKK NVLWDENNMS EYLTNPKKYI PGTKMAFGGL KKEKDRNDLI TYLKKACESolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CcP | [U-2H; U-15N] | protein | 0.4 mM |
2 | Cc | natural abundance | protein | 0.4 mM |
3 | Cc1 | natural abundance | protein | 0.4 mM |
4 | sodium phosphate | natural abundance | buffer | 20 mM |
5 | H2O | natural abundance | solvent | 93 % |
6 | D2O | [U-2H] | solvent | 7 % |