BMRB: 25561

Structure and assembly of the mouse ASC filament by combined NMR spectroscopy and cryo-electron microscopy

Authors

Sborgi, L.

Assembly

ASC PYD

Entity

1. ASC PYD (polymer, Thiol state: not present), 103 monomers, 11720.26 Da Detail

MGRARDAILD ALENLSGDEL KKFKMKLLTV QLREGYGRIP RGALLQMDAI DLTDKLVSYY LESYGLELTM TVLRDMGLQE LAEQLQTTKE EGSGSLEHHH HHH

Formula weight

11720.26 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 34.1 %, Completeness (bb): 64.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	34.1 % (410 of 1201)	18.2 % (114 of 625)	41.9 % (196 of 468)	92.6 % (100 of 108)
Backbone	64.3 % (396 of 616)	48.1 % (103 of 214)	64.3 % (193 of 300)	98.0 % (100 of 102)
Sidechain	15.6 % (106 of 679)	2.7 % (11 of 411)	36.3 % (95 of 262)	0.0 % (0 of 6)
Aromatic	0.0 % (0 of 66)	0.0 % (0 of 33)	0.0 % (0 of 33)
Methyl	9.7 % (12 of 124)	6.5 % (4 of 62)	12.9 % (8 of 62)

1. ASC PYD

MGRARDAILD ALENLSGDEL KKFKMKLLTV QLREGYGRIP RGALLQMDAI DLTDKLVSYY LESYGLELTM TVLRDMGLQE LAEQLQTTKE EGSGSLEHHH HHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 3.7

#	Name	Isotope labeling	Type	Concentration
1	ASC PYD	[U-100% 13C; U-100% 15N]		0.3 mM

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LACS Plot; CA

Referencing offset: -0.22 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.22 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
13C	water	protons	0.0 ppm	internal	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Release date

2016-06-29

Citation

Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy
Sborgi, L., Ravotti, F., Dandey, V.P., Dick, M.S., Mazur, A., Reckel, S., Chami, M., Scherer, S., Huber, M., Bockmann, A., Egelman, E.H., Stahlberg, H., Broz, P., Meier, B.H., Hiller, S.
Proc. Natl. Acad. Sci. U. S. A. (2015), 112, 13237-13242, PubMed 26464513 , DOI 10.1073/pnas.1507579112 , Abstract

Related entities 1. ASC PYD, : 1 : 1 : 87 entities Detail

Interaction partners 1. ASC PYD, : 10 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail