NMR Structure of the Myristylated Feline Immunodeficiency Virus Matrix Protein
XGNGASRDWK MAIKRCSNVA VGVGGKSKKF GEGNFRWAIR MANVSTGREP GDIPETLDQL RLVICDLQER REKFGSSKEI DMAIVTLKVF AVAGLLNMTV STAAAAENMY SQMGLDTRPS MKEAGGKEEG PPQAY
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 64.3 % (967 of 1505) | 62.6 % (493 of 787) | 60.7 % (350 of 577) | 87.9 % (124 of 141) |
Backbone | 79.0 % (627 of 794) | 91.4 % (254 of 278) | 65.1 % (252 of 387) | 93.8 % (121 of 129) |
Sidechain | 53.9 % (447 of 830) | 47.0 % (239 of 509) | 66.3 % (205 of 309) | 25.0 % (3 of 12) |
Aromatic | 41.2 % (33 of 80) | 40.0 % (16 of 40) | 39.5 % (15 of 38) | 100.0 % (2 of 2) |
Methyl | 81.8 % (108 of 132) | 78.8 % (52 of 66) | 84.8 % (56 of 66) |
1. Feline Immunodeficiency Virus Matrix Protein
XGNGASRDWK MAIKRCSNVA VGVGGKSKKF GEGNFRWAIR MANVSTGREP GDIPETLDQL RLVICDLQER REKFGSSKEI DMAIVTLKVF AVAGLLNMTV STAAAAENMY SQMGLDTRPS MKEAGGKEEG PPQAYSolvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 293 K, pH 7, Details 50mM sodium phosphate, 150mM sodium chloride, 10mM DTT
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium phosphate | natural abundance | 50 mM | |
2 | sodium chloride | natural abundance | 150 mM | |
3 | DTT | natural abundance | 10 mM | |
4 | ammonium chloride | [U-99% 15N] | 1 g/L | |
5 | glucose | natural abundance | 4 g/L | |
6 | MYRISTIC ACID | natural abundance | 0.5 mg/mL | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % | |
9 | Feline Immunodeficiency Virus Matrix Protein | [U-90% 15N] | 0.0 ~ 0.0 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7, Details 50mM sodium phosphate, 150mM sodium chloride, 10mM DTT
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | sodium phosphate | natural abundance | 50 mM | |
11 | sodium chloride | natural abundance | 150 mM | |
12 | DTT | natural abundance | 10 mM | |
13 | glucose | [U-100% 13C] | 4 g/L | |
14 | ammonium chloride | natural abundance | 1 g/L | |
15 | MYRISTIC ACID | natural abundance | 0.5 mg/mL | |
16 | D2O | natural abundance | 100 % | |
17 | Feline Immunodeficiency Virus Matrix Protein | [U-100% 13C] | 0.0 ~ 0.0 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7, Details 50mM sodium phosphate, 150mM sodium chloride, 10mM DTT
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
18 | sodium phosphate | natural abundance | 50 mM | |
19 | sodium chloride | natural abundance | 150 mM | |
20 | DTT | natural abundance | 10 mM | |
21 | glucose | [U-100% 13C] | 4 g/L | |
22 | ammonium chloride | natural abundance | 1 g/L | |
23 | MYRISTIC ACID | natural abundance | 0.5 mg/mL | |
24 | D2O | natural abundance | 100 % | |
25 | Feline Immunodeficiency Virus Matrix Protein | [U-100% 13C] | 0.0 ~ 0.0 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7, Details 50mM sodium phosphate, 150mM sodium chloride, 10mM DTT
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
26 | sodium phosphate | natural abundance | 50 mM | |
27 | sodium chloride | natural abundance | 150 mM | |
28 | DTT | natural abundance | 10 mM | |
29 | glucose | [U-100% 13C] | 4 g/L | |
30 | ammonium chloride | natural abundance | 1 g/L | |
31 | MYRISTIC ACID | natural abundance | 0.5 mg/mL | |
32 | D2O | natural abundance | 100 % | |
33 | Feline Immunodeficiency Virus Matrix Protein | [U-100% 13C] | 0.0 ~ 0.0 mM |
Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 293 K, pH 7, Details 50mM sodium phosphate, 150mM sodium chloride, 10mM DTT
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
34 | sodium phosphate | natural abundance | 50 mM | |
35 | sodium chloride | natural abundance | 150 mM | |
36 | DTT | natural abundance | 10 mM | |
37 | ammonium chloride | [U-99% 15N] | 1 g/L | |
38 | glucose | natural abundance | 4 g/L | |
39 | MYRISTIC ACID | natural abundance | 0.5 mg/mL | |
40 | H2O | natural abundance | 90 % | |
41 | D2O | natural abundance | 10 % | |
42 | Feline Immunodeficiency Virus Matrix Protein | [U-90% 15N] | 0.0 ~ 0.0 mM |