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BMRB: 25584

2N23

NMR structure of the complex between the PH domain of the Tfb1 subunit from TFIIH and the N-terminal activation domain of EKLF (TAD1)

Authors

Lecoq, L., Morse, T., Raiola, L., Arseneault, G., Omichinski, J.

Assembly

complex between the PH domain of the Tfb1 subunit from TFIIH and the N-terminal activation domain of EKLF (TAD1)

Entity

1. RNA polymerase II transcription factor B subunit 1 (polymer, Thiol state: not present), 115 monomers, 12883.54 Da Detail

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDAD

2. N-terminal transactivation domain of Krueppel-like factor 1 (polymer, Thiol state: not present), 19 monomers, 2357.466 Da Detail

DTQDDFLKWW RSEEAQDMG

Total weight

15241.006 Da

Max. entity weight

12883.54 Da

Source organism

Saccharomyces cerevisiae , Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.1 %, Completeness (bb): 95.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.1 % (1491 of 1567)	95.4 % (783 of 821)	93.9 % (565 of 602)	99.3 % (143 of 144)
Backbone	95.2 % (756 of 794)	97.8 % (263 of 269)	92.2 % (365 of 396)	99.2 % (128 of 129)
Sidechain	95.6 % (861 of 901)	94.2 % (520 of 552)	97.6 % (326 of 334)	100.0 % (15 of 15)
Aromatic	92.7 % (89 of 96)	89.6 % (43 of 48)	95.6 % (43 of 45)	100.0 % (3 of 3)
Methyl	98.5 % (130 of 132)	97.0 % (64 of 66)	100.0 % (66 of 66)

1. RNA polymerase II transcription factor B subunit 1

PSHSGAAIFE KVSGIIAINE DVSPAELTWR STDGDKVHTV VLSTIDKLQA TPASSEKMML RLIGKVDESK KRKDNEGNEV VPKPQRHMFS FNNRTVMDNI KMTLQQIISR YKDAD

2. N-terminal transactivation domain of Krueppel-like factor 1

DTQDDFLKWW RSEEAQDMG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5, Details Labeled Tfb1 + 3eq EKLF TAD1

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
2	entity_2	natural abundance	2 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5, Details Labeled Tfb1 + 3eq EKLF TAD1

#	Name	Isotope labeling	Concentration
5	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
6	entity_2	natural abundance	2 mM
7	D2O	natural abundance	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5, Details Labeled EKLF TAD1 + 3 eq Tfb1

#	Name	Isotope labeling	Concentration
8	entity_2	[U-100% 13C; U-100% 15N]	0.8 mM
9	entity_1	natural abundance	2 mM
10	H2O	natural abundance	90 %
11	D2O	natural abundance	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 300 K, pH 6.5, Details Labeled EKLF TAD1 + 3 eq Tfb1

#	Name	Isotope labeling	Concentration
12	entity_2	[U-100% 13C; U-100% 15N]	0.8 mM
13	entity_1	natural abundance	2 mM
14	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N23, Strand ID: A, B Detail

Release date

2016-04-24

Citation

Structural Characterization of the Complex between the N-terminal Transactivation Domain of EKLF and the p62/Tfb1 subunit of TFIIH
Morse, T., Lecoq, L., Raiola, L., Arseneault, G., Omichinski, J.G.
Not known

Related entities 1. RNA polymerase II transcription factor B subunit 1, : 2 : 4 entities Detail

Related entities 2. N-terminal transactivation domain of Krueppel-like factor 1, : 1 : 3 entities Detail

Interaction partners 1. RNA polymerase II transcription factor B subunit 1, : 49 interactors Detail

More details for 49 interactors identified by 12 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC