Tom1 negatively modulates binding of Tollip to phosphatidylinositol 3-phosphate via a coupled folding and binding mechanism
GPLGSEQIGK LRSELEMVSG NVRVMSEMLT ELVPTQAEPA DLELLQELNR TCRAMQQRVL ELIPQIANEQ LTEELLIVND NLNNVFLRHE RFERFRTGQT
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 39.0 % (463 of 1186) | 17.1 % (107 of 624) | 59.4 % (268 of 451) | 79.3 % (88 of 111) |
Backbone | 75.2 % (445 of 592) | 46.3 % (93 of 201) | 89.5 % (264 of 295) | 91.7 % (88 of 96) |
Sidechain | 15.4 % (106 of 689) | 3.8 % (16 of 423) | 35.9 % (90 of 251) | 0.0 % (0 of 15) |
Aromatic | 0.0 % (0 of 34) | 0.0 % (0 of 17) | 0.0 % (0 of 17) | |
Methyl | 6.3 % (8 of 128) | 4.7 % (3 of 64) | 7.8 % (5 of 64) |
1. entity
GPLGSEQIGK LRSELEMVSG NVRVMSEMLT ELVPTQAEPA DLELLQELNR TCRAMQQRVL ELIPQIANEQ LTEELLIVND NLNNVFLRHE RFERFRTGQTSolvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Tom1 GAT | [U-99% 13C; U-99% 15N] | 0.9 mM | |
2 | Tollip TBD | natural abundance | 1.1 mM | |
3 | DSS | natural abundance | 50 uM | |
4 | potassium chloride | natural abundance | 50 mM | |
5 | TRIS | [U-2H] | 20 mM | |
6 | sodium azide | natural abundance | 1 mM | |
7 | DTT | [U-2H] | 1 mM |