BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	91.9 % (1067 of 1161)	90.9 % (560 of 616)	92.1 % (410 of 445)	97.0 % (97 of 100)
Backbone	96.5 % (529 of 548)	96.2 % (178 of 185)	96.7 % (264 of 273)	96.7 % (87 of 90)
Sidechain	89.2 % (626 of 702)	88.6 % (382 of 431)	89.7 % (234 of 261)	100.0 % (10 of 10)
Aromatic	71.4 % (90 of 126)	76.2 % (48 of 63)	65.6 % (40 of 61)	100.0 % (2 of 2)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. entity

GSHMMATSDV KPKSISRAKK WSEEIENLYR FQQAGYRDEI EYKQVKQVAM VDRWPETGYV KKLQRRDNTF FYYNKERECE DKEVHKVKVY VY

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N2Y, Strand ID: A Detail

Release date

2016-01-18

Citation

Dissecting the structural basis of MEIG1 interaction with PACRG
Li, W., Walavalkar, N.M., Buchwald, W.A., Teves, M.E., Zhang, L., Liu, J., Bilinovich, S., Peterson, D., Strauss, J.F., Williams, D.C., Zhang, Z.
Sci. Rep. (2016), 6, 18278-18278, PubMed 26726850 , DOI 10.1038/srep18278 , Abstract

Related entities 1. meiosis-expressed gene 1 (Meig1), : 2 : 10 entities Detail

Interaction partners 1. meiosis-expressed gene 1 (Meig1), : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

3 3D C(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

4 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

5 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

6 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

7 3D HBHA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

10 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

11 3D H(CCO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

12 2D IPAP-HSQC

Instrument

Bruker Avance - 850 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

13 2D IPAP-HSQC

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

14 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	Meig1	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_25627_2n2y.nef
Input source #2: Coordindates	2n2y.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

------------10--------20--------30--------40--------50--------60--------70--------80--------
GSHMMATSDVKPKSISRAKKWSEEIENLYRFQQAGYRDEIEYKQVKQVAMVDRWPETGYVKKLQRRDNTFFYYNKERECEDKEVHKVKVYVY
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSHMMATSDVKPKSISRAKKWSEEIENLYRFQQAGYRDEIEYKQVKQVAMVDRWPETGYVKKLQRRDNTFFYYNKERECEDKEVHKVKVYVY
--------10--------20--------30--------40--------50--------60--------70--------80--------90--

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	92	0	0	100.0

Content subtype: combined_25627_2n2y.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1062		97.8 (chain: A, length: 92)
1	Distance restraints	CNS/XPLOR_distance_constraints_4	Warning	1623 (1)	noe	95.7 (chain: A, length: 92)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	172 (172)	.	94.6 (chain: A, length: 92)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_2	OK	104 (104)	.	79.3 (chain: A, length: 92)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 97.8%
- Total number of assignments
  - ¹H chemical shifts: 553
  - ¹³C chemical shifts: 410
  - ¹⁵N chemical shifts: 99
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	616	553	89.8
¹³C chemical shifts	445	410	92.1
¹⁵N chemical shifts	107	99	92.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	185	178	96.2
¹³C chemical shifts	184	176	95.7
¹⁵N chemical shifts	90	87	96.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	431	375	87.0
¹³C chemical shifts	261	234	89.7
¹⁵N chemical shifts	17	12	70.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	38	35	92.1
¹³C chemical shifts	38	35	92.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	63	48	76.2
¹³C chemical shifts	61	40	65.6
¹⁵N chemical shifts	2	2	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_4
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 95.7%
- Total number of restraints: 1620
- Weight of restraints: 1.0 (1620)
- Potential type of restraints: square-well-parabolic (1620)
- Range of distance target values: 2.08, 3.65 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 94.6%
- Total number of restraints: 172
- Total number of restraints per polymer type: 172
- Weight of restraints: 1.0 (172)
- Potential type of restraints: square-well-parabolic (172)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 79.3%
- Total number of restraints: 104
- Potential type of restraints: undefined (104)
- Range of observed RDC values: 100.0, -100.0 (Hz)
- RDC restraints per residue
  - Chain_ID: A
    None

Keywords PROTEIN

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Found 1 Document (0.656 seconds)

BMRB: 25627

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. meiosis-expressed gene 1 (Meig1), : 2 : 10 entities Detail

Interaction partners 1. meiosis-expressed gene 1 (Meig1), : 1 interactors Detail

Experiments performed 14 experiments Detail

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NMR combined restraints 5 contents Detail