SOLUTION STRUCTURE OF THE LINK MODULE OF HUMAN TSG-6 IN PRESENCE OF A CHONDROITIN 4-SULFATE HEXASACCHARIDE
GVYHREARSG KYKLTYAEAK AVCEFEGGHL ATYKQLEAAR KIGFHVCAAG WMAKGRVGYP IVKPGPNCGF GKTGIIDYGI RLNRSERWDA YCYNPHAK
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS23:SG | 1:CYS92:SG |
2 | disulfide | sing | 1:CYS47:SG | 1:CYS68:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.3 % (1015 of 1136) | 95.8 % (567 of 592) | 80.0 % (355 of 444) | 93.0 % (93 of 100) |
Backbone | 94.1 % (546 of 580) | 96.1 % (197 of 205) | 93.2 % (262 of 281) | 92.6 % (87 of 94) |
Sidechain | 86.3 % (553 of 641) | 95.6 % (370 of 387) | 71.4 % (177 of 248) | 100.0 % (6 of 6) |
Aromatic | 44.0 % (59 of 134) | 85.1 % (57 of 67) | 0.0 % (0 of 65) | 100.0 % (2 of 2) |
Methyl | 97.7 % (84 of 86) | 95.3 % (41 of 43) | 100.0 % (43 of 43) |
1. Link-TSG-6
GVYHREARSG KYKLTYAEAK AVCEFEGGHL ATYKQLEAAR KIGFHVCAAG WMAKGRVGYP IVKPGPNCGF GKTGIIDYGI RLNRSERWDA YCYNPHAKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MES | natural abundance | 50 mM | |
2 | NaN3 | natural abundance | 0.02 % | |
3 | Link-TSG-6 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
4 | CHONDROITIN 4-SULFATE HEXASACCHARIDE | natural abundance | 0.6 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details polyacrylamide neutral gel for an alignment media
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | MES | natural abundance | 50 mM | |
6 | NaN3 | natural abundance | 0.02 % | |
7 | Link-TSG-6 | [U-100% 13C; U-100% 15N] | 0.5 mM | |
8 | CHONDROITIN 4-SULFATE HEXASACCHARIDE | natural abundance | 1.0 mM |