Solution Structure of the Q343R Mutant of TDP-43 Amyloidogenic Core Region
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 51.0 % (277 of 543) | 43.9 % (125 of 285) | 56.4 % (110 of 195) | 66.7 % (42 of 63) |
Backbone | 79.7 % (236 of 296) | 80.8 % (84 of 104) | 76.4 % (110 of 144) | 87.5 % (42 of 48) |
Sidechain | 26.5 % (77 of 291) | 22.7 % (41 of 181) | 37.9 % (36 of 95) | 0.0 % (0 of 15) |
Aromatic | 9.4 % (3 of 32) | 18.8 % (3 of 16) | 0.0 % (0 of 15) | 0.0 % (0 of 1) |
Methyl | 46.4 % (13 of 28) | 42.9 % (6 of 14) | 50.0 % (7 of 14) |
1. entity
MNFGAFSINP AMMAAAQAAL QSSWGMMGML ASRQNQSGPS GNNQNQGNMQSolvent system 92% H2O/8% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | GB1-TDP(311-360)-Q343R | [U-99% 13C; U-99% 15N] | 600 uM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 w/v | |
5 | D2O | [U-99% 2H] | 8 % | |
6 | H2O | natural abundance | 92 % |