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FBP28 WW L453W
Authors
Medina, J., Macias, M.J., Martin-Malpartida, P., Scheraga, H.A.
Assembly
L453W
Entity
1. L453W (polymer, Thiol state: not present), 37 monomers, 4432.720 Da Detail

GATAVSEWTE YKTADGKTYY YNNRTWESTW EKPQELK


Formula weight
4432.72 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 89.2 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

Total1H
All89.2 % (206 of 231)89.2 % (206 of 231)
Backbone98.7 % (74 of 75)98.7 % (74 of 75)
Sidechain84.6 % (132 of 156)84.6 % (132 of 156)
Aromatic76.5 % (26 of 34)76.5 % (26 of 34)
Methyl100.0 % (13 of 13)100.0 % (13 of 13)

1. entity

GATAVSEWTE YKTADGKTYY YNNRTWESTW EKPQELK

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 5.8


#NameIsotope labelingTypeConcentration
1entitynatural abundance500.0 ~ 1000.0 uM
2sodium phosphatenatural abundance25 mM
3sodium chloridenatural abundance100 mM
4H2Onatural abundance90 %
5D2Onatural abundance10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2N4T, Strand ID: A Detail


Release date
2015-10-18
Citation
Preventing fibril formation of a protein by selective mutation
Maisuradze, G.G., Medina, J., Kachlishvili, K., Krupa, P., Mozolewska, M., Martin-Malpartida, P., Maisuradze, L., Macias, M.J., Scheraga, H.A.
Proc. Natl. Acad. Sci. U. S. A. (2015), 112, 13549-13554, PubMed 26483482 , DOI 10.1073/pnas.1518298112 ,
Related entities 1. L453W, : 1 : 17 : 113 entities Detail
Interaction partners 1. L453W, : 7 interactors Detail
Experiments performed 2 experiments Detail
NMR combined restraints 3 contents Detail
Keywords NMR, Variants, WW domain