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FBP28 WW E454Y
Authors
Medina, J., Macias, M., Martin-Malpartida, P., Scheraga, H.A.
Assembly
E454Y
Entity
1. E454Y (polymer, Thiol state: not present), 37 monomers, 4393.728 Da Detail

GATAVSEWTE YKTADGKTYY YNNRTLYSTW EKPQELK


Formula weight
4393.728 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.3 %, Completeness: 44.7 %, Completeness (bb): 34.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All44.7 % (199 of 445)73.5 % (169 of 230)14.9 % (26 of 174) 9.8 % (4 of 41)
Backbone34.1 % (75 of 220)76.0 % (57 of 75)12.8 % (14 of 109)11.1 % (4 of 36)
Sidechain48.8 % (127 of 260)72.3 % (112 of 155)15.0 % (15 of 100) 0.0 % (0 of 5)
Aromatic32.8 % (21 of 64)59.4 % (19 of 32) 6.7 % (2 of 30) 0.0 % (0 of 2)
Methyl63.3 % (19 of 30)93.3 % (14 of 15)33.3 % (5 of 15)

1. entity

GATAVSEWTE YKTADGKTYY YNNRTLYSTW EKPQELK

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 285 K, pH 5.8


#NameIsotope labelingTypeConcentration
1entitynatural abundance500.0 ~ 1000.0 uM
2sodium phosphatenatural abundance25 mM
3sodium chloridenatural abundance100 mM
4H2Onatural abundance90 %
5D2Onatural abundance10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2N4U, Strand ID: A Detail


Release date
2015-10-18
Citation
Preventing fibril formation of a protein by selective mutation
Maisuradze, G.G., Medina, J., Kachlishvili, K., Krupa, P., Mozolewska, M., Martin-Malpartida, P., Maisuradze, L., Macias, M., Scheraga, H.A.
Proc. Natl. Acad. Sci. U. S. A. (2015), 112, 13549-13554, PubMed 26483482 , DOI 10.1073/pnas.1518298112 ,
Related entities 1. E454Y, : 1 : 17 : 118 entities Detail
Interaction partners 1. E454Y, : 20 interactors Detail
Experiments performed 2 experiments Detail
NMR combined restraints 3 contents Detail
Keywords NMR, Variants, WW domain