BMRB: 25692

SOLUTION STRUCTURE OF OVIS ARIES PRP

Authors

Munoz, C., Egalon, A., Beringue, V., Rezaei, H., Dron, M., Sizun, C.

Assembly

OVIS ARIES PRP

Entity

1. OVIS ARIES PRP (polymer, Thiol state: all disulfide bound), 206 monomers, 23553.07 Da Detail

MGSSHHHHHH SSGLVPRGSH MSNKPSKPKT NMKHVAGAAA AGAVVGGLGG YMLGSVMSRP LIHFGNDYED RYYRENMYRY PNQVYYRPVD QYSNQNNFVH DCVNITVKQH TVTTTTKGEN FTETDIKIME RVVEQMCITQ YQRESQAYYQ RGASVNITVK QHTVTTTTKG ENFTETDIKI MERVVEQMCI TQYQRESQAY YQRGAS

Formula weight

23553.07 Da

Entity Connection

disulfide 1 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS102:SG	1:CYS137:SG

Source organism

Ovis aries

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 70.4 %, Completeness: 60.8 %, Completeness (bb): 68.3 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	15N
All	60.8 % (890 of 1463)	59.5 % (736 of 1236)	67.8 % (154 of 227)
Backbone	68.3 % (424 of 621)	68.2 % (287 of 421)	68.5 % (137 of 200)
Sidechain	55.5 % (467 of 842)	55.2 % (450 of 815)	63.0 % (17 of 27)
Aromatic	55.8 % (58 of 104)	55.8 % (58 of 104)
Methyl	67.0 % (61 of 91)	67.0 % (61 of 91)

1. entity

MGSSHHHHHH SSGLVPRGSH MSNKPSKPKT NMKHVAGAAA AGAVVGGLGG YMLGSVMSRP LIHFGNDYED RYYRENMYRY PNQVYYRPVD QYSNQNNFVH DCVNITVKQH TVTTTTKGEN FTETDIKIME RVVEQMCITQ YQRESQAYYQ RGASVNITVK QHTVTTTTKG ENFTETDIKI MERVVEQMCI TQYQRESQAY YQRGAS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298.000 (±0.1) K, pH 5.300 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PrPWT	[U-99% 15N]		0.3 (±3.0E-5) mM
2	Sodium acetate	natural abundance		10.0 (±0.001) mM
3	H2O	natural abundance		90 (±0.001) %
4	D2O	natural abundance		10 (±0.001) %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2N53, Strand ID: A Detail

---

Release date

2016-05-22

Citation

Generating bona fide mammalian prions with internal deletions
Munoz-Montesino, C., Sizun, C., Moudjou, M., Herzog, L., Reine, F., Chapuis, J., Ciric, D., Igel-Egalon, A., Laude, H., Beringue, V., Rezaei, H., Dron, M.
J. Virol. (2016), 90, 6963-6975, PubMed 27226369 , DOI 10.1128/JVI.00555-16 , Abstract

Related entities 1. OVIS ARIES PRP, : 3 entities Detail

Experiments performed 5 experiments Detail