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C-terminal domain of Cdc37 cochaperone
Authors
Keramisanou, D., Dudhat, A., Pare, M.
Assembly
C-terminal domain of Cdc37 cochaperone
Entity
1. C-terminal domain of Cdc37 cochaperone (polymer), 94 monomers, 10186.27 Da Detail

GHMGPGGLDP VEVYESLPEE LQKCFDVKDV QMLQDAISKM DPTDAKYHMQ RCIDSGLWVP NSKASEAKEG EEAGPGDPLL EAVPKTGDEK DVSV


Formula weight
10186.27 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.7 %, Completeness: 89.7 %, Completeness (bb): 89.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All89.7 % (935 of 1042)90.5 % (494 of 546)87.9 % (355 of 404)93.5 % (86 of 92)
Backbone89.4 % (490 of 548)89.9 % (170 of 189)88.3 % (241 of 273)91.9 % (79 of 86)
Sidechain88.8 % (514 of 579)89.6 % (320 of 357)87.0 % (188 of 216)100.0 % (6 of 6)
Aromatic60.9 % (28 of 46)65.2 % (15 of 23)59.1 % (13 of 22) 0.0 % (0 of 1)
Methyl100.0 % (84 of 84)100.0 % (42 of 42)100.0 % (42 of 42)

1. Cdc37

GHMGPGGLDP VEVYESLPEE LQKCFDVKDV QMLQDAISKM DPTDAKYHMQ RCIDSGLWVP NSKASEAKEG EEAGPGDPLL EAVPKTGDEK DVSV

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5, Details 50mM Hepes, pH=7.5, 100mM NaCl, 2mM DTT


#NameIsotope labelingTypeConcentration
1Cdc37[U-100% 13C; U-100% 15N]0.3 ~ 0.5 mM
2sodium chloridenatural abundance100 mM
3HEPESnatural abundance50 mM
4D2O[U-100% 2H]7 %
5H2Onatural abundance93 %
6DTTnatural abundance2 mM
Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5, Details 50mM Hepes, pH=7.5, 100mM NaCl, 2mM DTT


#NameIsotope labelingTypeConcentration
7Cdc37[U-100% 15N]0.3 ~ 0.5 mM
8sodium chloridenatural abundance100 mM
9HEPESnatural abundance50 mM
10D2O[U-2H]7 %
11H2Onatural abundance93 %
12DTTnatural abundance2 mM
Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5, Details 50mM Hepes, pH=7.5, 100mM NaCl, 2mM DTT


#NameIsotope labelingTypeConcentration
13Cdc37[U-10% 13C; U-100% 15N]0.3 ~ 0.5 mM
14sodium chloridenatural abundance100 mM
15HEPESnatural abundance5 mM
16D2O[U-2H]7 %
17H2Onatural abundance93 %
18DTTnatural abundance2 mM

LACS Plot; CA
Referencing offset: -0.17 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.17 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: 0.02 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.04 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 2N5X, Strand ID: A Detail


Release date
2015-10-04
Citation
The C-terminal domain of human Cdc37 studied by solution NMR
Zhang, Z., Keramisanou, D., Dudhat, A., Pare, M., Gelis, I.
J. Biomol. NMR (2015), 63, 315-321, PubMed 26400850 , DOI 10.1007/s10858-015-9988-6 ,
Related entities 1. C-terminal domain of Cdc37 cochaperone, : 1 : 13 entities Detail
Interaction partners 1. C-terminal domain of Cdc37 cochaperone, : 258 interactors Detail
Experiments performed 14 experiments Detail
NMR combined restraints 6 contents Detail
Keywords Hsp90, cdc37, co-chaperone, kinase