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BMRB: 25748


25748
2N62
ddFLN5+110
Authors
Cabrita, L.D., Cassaignau, A.ME., Launay, H.MM., Waudby, C.A., Camilloni, C., Robertson, A.L., Wang, X., Wlodarski, T., Wentink, A.S., Vendruscolo, M., Dobson, C.M., Christodoulou, J.
Assembly
ddFLN5+110
Entity
1. ddFLN5+110 (polymer), 221 monomers, 23394.47 Da Detail

HHHHHHASKP APSAEHSYAE GEGLVKVFDN APAEFTIFAV DTKGVARTDG GDPFEVAING PDGLVVDAKV TDNNDGTYGV VYDAPVEGNY NVNVTLRGNP IKNMPIDVKC IEGANGEDSS FGSFTFTVAA KNKKGEVKTY GGDKFEVSIT GPAEEITLDA IDNQDGTYTA AYSLVGNGRF STGVKLNGKH IEGSPFKQVL GNELFSTPVW ISQAQGIRAG P


Formula weight
23394.47 Da
Source organism
Escherichia coli
Exptl. method
solution NMR
Refine. method
molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 68.3 %, Completeness: 29.5 %, Completeness (bb): 49.4 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All29.5 % (713 of 2414)21.3 % (263 of 1237)33.9 % (321 of 948)56.3 % (129 of 229)
Backbone49.4 % (643 of 1302)47.0 % (215 of 457)47.3 % (301 of 636)60.8 % (127 of 209)
Sidechain12.0 % (157 of 1306) 5.9 % (46 of 780)21.7 % (110 of 506) 5.0 % (1 of 20)
Aromatic 3.8 % (8 of 210) 3.8 % (4 of 105) 3.8 % (4 of 104) 0.0 % (0 of 1)
Methyl13.9 % (32 of 230)11.3 % (13 of 115)16.5 % (19 of 115)

1. entity

HHHHHHASKP APSAEHSYAE GEGLVKVFDN APAEFTIFAV DTKGVARTDG GDPFEVAING PDGLVVDAKV TDNNDGTYGV VYDAPVEGNY NVNVTLRGNP IKNMPIDVKC IEGANGEDSS FGSFTFTVAA KNKKGEVKTY GGDKFEVSIT GPAEEITLDA IDNQDGTYTA AYSLVGNGRF STGVKLNGKH IEGSPFKQVL GNELFSTPVW ISQAQGIRAG P

Show sample condition
Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5


#NameIsotope labelingTypeConcentration
1ddFLN5+110[U-15N]10 uM
2HEPESnatural abundance10 mM
3ammonium chloridenatural abundance30 mM
4MgCl2natural abundance12 mM
5BMEnatural abundance2 mM
6EDTAnatural abundance1 mM
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5


#NameIsotope labelingTypeConcentration
7ddFLN5+110[U-100% 2H, Ile d1-13CH3]10 uM
8HEPES[U-2H]10 mM
9ammonium chloride[U-2H]30 mM
10MgCl2[U-2H]12 mM
11BME[U-2H]2 mM
12EDTA[U-2H]1 mM

LACS Plot; CA
Referencing offset: -0.46 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.46 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.07 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 1.2 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 3 models in PDB: 2N62, Strand ID: L Detail

Release date
2016-02-28
Citation
A strategy for co-translational folding studies of ribosome-bound nascent chain complexes using NMR spectroscopy
Cassaignau, A.ME., Launay, H.MM., Karyadi, M., Wang, X., Waudby, C.A., Deckert, A., Robertson, A.L., Christodoulou, J., Cabrita, L.D.
Nat. Protoc. (2016), 11, 1492-1507, PubMed 27466710 , DOI 10.1038/nprot.2016.101 ,
Related entities 1. ddFLN5+110, : 1 : 4 : 155 entities Detail
More details for 160 related entities
Experiments performed 2 experiments Detail
nullKeywords protein