BMRB: 25777

Solution NMR structure of Tetrahymena telomerase RNA pseudoknot

Authors

Cash, D.D., Feigon, J.

Assembly

Tetrahymena telomerase RNA pseudoknot

Entity

1. Tetrahymena telomerase RNA pseudoknot (polymer, Thiol state: not present), 31 monomers, 9871.909 Da Detail

XACCUUCACC AAUUAGGUUC AAAUAAGUGG U

Formula weight

9871.909 Da

Source organism

Tetrahymena thermophila

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 52.4 %, Completeness (bb): 37.3 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C
All	52.4 % (244 of 466)	64.0 % (167 of 261)	37.6 % (77 of 205)
Suger, PO4	37.3 % (123 of 330)	50.6 % (91 of 180)	21.3 % (32 of 150)
Nucleobase	89.0 % (121 of 136)	93.8 % (76 of 81)	81.8 % (45 of 55)
Aromatic	89.5 % (111 of 124)	95.7 % (66 of 69)	81.8 % (45 of 55)

1. RNA (31-MER)

XACCUUCACC AAUUAGGUUC AAAUAAGUGG U

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	RNA (31-MER)	natural abundance		10 mM
2	sodium phosphate	natural abundance		10 mM
3	KCl	natural abundance		50 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
6	RNA (31-MER)	natural abundance		10 mM
7	sodium phosphate	natural abundance		10 mM
8	KCl	natural abundance		50 mM
9	D2O	natural abundance		100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
10	RNA (31-MER)	natural abundance		10 mM
11	sodium phosphate	natural abundance		10 mM
12	KCl	natural abundance		50 mM
13	ADENOSINE-5'-MONOPHOSPHATE	[U-13C; U-15N]		100 %
14	URIDINE-5'-MONOPHOSPHATE	[U-13C; U-15N]		100 %
15	H2O	natural abundance		90 %
16	D2O	natural abundance		10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
17	RNA (31-MER)	natural abundance		10 mM
18	sodium phosphate	natural abundance		10 mM
19	KCl	natural abundance		50 mM
20	CYTIDINE-5'-MONOPHOSPHATE	[U-13C; U-15N]		100 %
21	GUANOSINE-5'-MONOPHOSPHATE	[U-13C; U-15N]		100 %
22	H2O	natural abundance		90 %
23	D2O	natural abundance		10 %

Sample #5

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
24	RNA (31-MER)	natural abundance		10 mM
25	sodium phosphate	natural abundance		10 mM
26	KCl	natural abundance		50 mM
27	CYTIDINE-5'-MONOPHOSPHATE	[U-13C; U-15N]		100 %
28	GUANOSINE-5'-MONOPHOSPHATE	[U-13C; U-15N]		100 %
29	ADENOSINE-5'-MONOPHOSPHATE	[U-13C; U-15N]		100 %
30	CYTIDINE-5'-MONOPHOSPHATE	[U-13C; U-15N]		100 %
31	H2O	natural abundance		90 %
32	D2O	natural abundance		10 %

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Release date

2015-10-25

Citation

Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions
Jiang, J., Chan, H., Cash, D.D., Miracco, E.J., Ogorzalek Loo, R.R., Cascio, D., O'Brien Johnson, R., Upton, H.E., Collins, K., Loo, J.A., Zhou, H., Feigon, J.
Science (2015), 350, aab4070-aab4070, PubMed 26472759 , DOI 10.1126/science.aab4070 , Abstract

Entries sharing articles EMDB: 2 entries Detail

Experiments performed 16 experiments Detail

Chemical shift validation 3 contents Detail