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BMRB: 25779

Partial 1H, 13C and 15N backbone resonance assignments for the carboxy-terminal RNA recognition motif of Plasmodium falciparum Ser/Arg-rich protein 1

Authors

Ganguly, A.K., Verma, G., Bhavesh, N.

Assembly

RRM2 monomer

Entity

1. RRM2 monomer (polymer, Thiol state: all free), 84 monomers, 9467.486 Da Detail

GSHMRRGRYV VEVTGLPISG SWQDLKDHLR EAGECGHADV FKDGTGEVSF FNKDDMLEAI DKFNGSIFRS HEGEKSKISI RQKK

Formula weight

9467.486 Da

Source organism

Plasmodium falciparum

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.7 %, Completeness: 29.1 %, Completeness (bb): 56.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	29.1 % (283 of 973)	14.5 % (74 of 511)	36.6 % (137 of 374)	81.8 % (72 of 88)
Backbone	56.0 % (281 of 502)	41.2 % (73 of 177)	56.6 % (137 of 242)	85.5 % (71 of 83)
Sidechain	11.9 % (65 of 545)	0.3 % (1 of 334)	30.6 % (63 of 206)	20.0 % (1 of 5)
Aromatic	2.3 % (2 of 86)	2.3 % (1 of 43)	0.0 % (0 of 42)	100.0 % (1 of 1)
Methyl	4.5 % (3 of 66)	0.0 % (0 of 33)	9.1 % (3 of 33)

1. RRM2

GSHMRRGRYV VEVTGLPISG SWQDLKDHLR EAGECGHADV FKDGTGEVSF FNKDDMLEAI DKFNGSIFRS HEGEKSKISI RQKK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.9

#	Name	Isotope labeling	Type	Concentration
1	RRM2	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	TCEP	natural abundance		3 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Release date

2015-08-27

Citation

The N-terminal RNA Recognition Motif of PfSR1 Confers Semi-specificity for Pyrimidines during RNA Recognition
Ganguly, A.K., Verma, G., Bhavesh, N.
J. Mol. Biol. (2018), PubMed 30500338 , DOI 10.1016/j.jmb.2018.11.020 , Abstract

Related entities 1. RRM2 monomer, : 1 : 65 entities Detail

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance III - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.9

#	Name	Isotope labeling	Type	Concentration
1	RRM2	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	TCEP	natural abundance		3 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance III - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.9

#	Name	Isotope labeling	Type	Concentration
1	RRM2	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	TCEP	natural abundance		3 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

3 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance III - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.9

#	Name	Isotope labeling	Type	Concentration
1	RRM2	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	TCEP	natural abundance		3 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

4 3D HNCACB

Instrument

Bruker Avance III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.9

#	Name	Isotope labeling	Type	Concentration
1	RRM2	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	TCEP	natural abundance		3 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

5 3D CBCA(CO)NH

Instrument

Bruker Avance III - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.9

#	Name	Isotope labeling	Type	Concentration
1	RRM2	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	TCEP	natural abundance		3 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

6 3D C(CO)NH

Instrument

Bruker Avance III - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.9

#	Name	Isotope labeling	Type	Concentration
1	RRM2	[U-100% 13C; U-100% 15N]		0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance		25 mM
3	sodium chloride	natural abundance		100 mM
4	TCEP	natural abundance		3 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr25779_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr25779_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	273		83.3 (chain: 1, length: 84)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 84, Sequence coverage: 83.3%
- Total number of assignments
  - ¹H chemical shifts: 71
  - ¹³C chemical shifts: 131
  - ¹⁵N chemical shifts: 71
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords Plasmodium falciparum, RNA recognition motif, Ser/Arg-rich protein, pseudo-RRM