BMRB: 25782

Astexin2-dC4

Authors

Link, A., Maksimov, M.O.

Assembly

Lasso peptide Astexin2-dC4

Entity

1. Lasso peptide Astexin2-dC4 (polymer, Thiol state: not present), 20 monomers, 2133.319 Da Detail

GLTQIQALDS VSGQFRDQLG

Formula weight

2133.319 Da

Source organism

Asticcacaulis excentricus

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 84.3 %, Completeness (bb): 75.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	84.3 % (167 of 198)	92.2 % (106 of 115)	73.5 % (61 of 83)
Backbone	75.0 % (75 of 100)	93.0 % (40 of 43)	61.4 % (35 of 57)
Sidechain	94.8 % (109 of 115)	91.7 % (66 of 72)	100.0 % (43 of 43)
Aromatic	100.0 % (10 of 10)	100.0 % (5 of 5)	100.0 % (5 of 5)
Methyl	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (12 of 12)

1. LASSO PEPTIDE ASTEXIN2-dC4

GLTQIQALDS VSGQFRDQLG

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 K

#	Name	Isotope labeling	Type	Concentration
1	astexin2-dC4	natural abundance		2.7 mg/mL

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 K

#	Name	Isotope labeling	Type	Concentration
2	astexin2-dC4	natural abundance		4.9 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 2N6U, Strand ID: A Detail

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Release date

2015-11-16

Citation

Elucidating the Specificity Determinants of the AtxE2 Lasso Peptide Isopeptidase
Maksimov, M.O., Koos, J.D., Zong, C., Link, A., Lisko, B.
J. Biol. Chem. (2015), 290, 30806-30812, PubMed 26534965 , DOI 10.1074/jbc.M115.694083 , Abstract

Related entities 1. Lasso peptide Astexin2-dC4, : 1 : 3 entities Detail

Experiments performed 5 experiments Detail