NMR structure of the prolactin receptor transmembrane domain
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 88.5 % (370 of 418) | 83.9 % (172 of 205) | 93.1 % (161 of 173) | 92.5 % (37 of 40) |
Backbone | 94.1 % (209 of 222) | 90.8 % (69 of 76) | 96.3 % (105 of 109) | 94.6 % (35 of 37) |
Sidechain | 84.8 % (196 of 231) | 79.8 % (103 of 129) | 91.9 % (91 of 99) | 66.7 % (2 of 3) |
Aromatic | 85.7 % (36 of 42) | 85.7 % (18 of 21) | 84.2 % (16 of 19) | 100.0 % (2 of 2) |
Methyl | 92.9 % (65 of 70) | 91.4 % (32 of 35) | 94.3 % (33 of 35) |
1. PRLRTMD
GSFTMNDTTV WISVAVLSAV ICLIIVWAVA LKGYSMVSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details DHPC micelles
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | PRLRTMD | [U-13C; U-15N] | 0.8 mM | |
2 | DSS | natural abundance | 2 mM | |
3 | sodium azide | natural abundance | 0.05 % | |
4 | DHPC | natural abundance | 560 mM | |
5 | sodium phosphate | natural abundance | 20 mM | |
6 | TCEP | natural abundance | 4 mM | |
7 | sodium chloride | natural abundance | 50 mM | |
8 | H2O | natural abundance | 90 % | |
9 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details DHPC micelles
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | PRLRTMD | [U-13C; U-15N] | 1 mM | |
11 | DSS | natural abundance | 2 mM | |
12 | sodium azide | natural abundance | 0.05 % | |
13 | DHPC | [U-2H] | 700 mM | |
14 | sodium phosphate | natural abundance | 20 mM | |
15 | TCEP | natural abundance | 4 mM | |
16 | sodium chloride | natural abundance | 50 mM | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | PRLRTMD | [U-15N] | 0.7 mM | |
20 | DSS | natural abundance | 2 mM | |
21 | sodium azide | natural abundance | 0.05 % | |
22 | DHPC | natural abundance | 490 mM | |
23 | sodium phosphate | natural abundance | 20 mM | |
24 | TCEP | natural abundance | 4 mM | |
25 | sodium chloride | natural abundance | 50 mM | |
26 | H2O | natural abundance | 90 % | |
27 | D2O | natural abundance | 10 % |