NMR-SAXS/WAXS Structure of the core of the U4/U6 di-snRNA
GGCCUUAUGC ACGGGAAAUA CGCAUAUCAG UGAGGAUUCG UCCGAGAUUG UGUUUUUGCU GGUGUAAAUC AGCAGUUCCC CUGCAUAAGG CU
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 4.1 % (60 of 1472) | 3.7 % (30 of 801) | 0.8 % (5 of 619) | 48.1 % (25 of 52) |
Suger, PO4 | 0.0 % (0 of 1012) | 0.0 % (0 of 552) | 0.0 % (0 of 460) | |
Nucleobase | 13.0 % (60 of 460) | 12.0 % (30 of 249) | 3.1 % (5 of 159) | 48.1 % (25 of 52) |
Aromatic | 14.2 % (60 of 422) | 14.2 % (30 of 211) | 3.1 % (5 of 159) | 48.1 % (25 of 52) |
1. RNA (92-MER)
GGCCUUAUGC ACGGGAAAUA CGCAUAUCAG UGAGGAUUCG UCCGAGAUUG UGUUUUUGCU GGUGUAAAUC AGCAGUUCCC CUGCAUAAGG CUSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA (92-MER) | [U-13C; U-15N]-Gua, -Ura | 0.6 mM | |
2 | potassium chloride | natural abundance | 0.02 mM | |
3 | potassium phosphate | natural abundance | 0.02 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | RNA (92-MER) | [U-13C; U-15N]-Ade | 0.7 mM | |
5 | potassium chloride | natural abundance | 0.02 mM | |
6 | potassium phosphate | natural abundance | 0.02 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | RNA (92-MER) | [U-13C; U-15N]-Gua, -Ura | 0.6 mM | |
8 | potassium chloride | natural abundance | 0.02 mM | |
9 | potassium phosphate | natural abundance | 0.02 mM | |
10 | Pf1 phage | natural abundance | 5 mg/mL |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | RNA (92-MER) | [U-13C; U-15N]-Ade | 0.7 mM | |
12 | potassium chloride | natural abundance | 0.02 mM | |
13 | potassium phosphate | natural abundance | 0.02 mM | |
14 | Pf1 phage | natural abundance | 5 mg/mL |