Structure of the transmembrane domain of human nicastrin in DPC micelles
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 57.3 % (359 of 627) | 60.8 % (192 of 316) | 47.3 % (122 of 258) | 84.9 % (45 of 53) |
Backbone | 78.8 % (252 of 320) | 80.7 % (88 of 109) | 75.5 % (120 of 159) | 84.6 % (44 of 52) |
Sidechain | 39.7 % (142 of 358) | 50.2 % (104 of 207) | 24.7 % (37 of 150) | 100.0 % (1 of 1) |
Aromatic | 2.9 % (2 of 70) | 5.7 % (2 of 35) | 0.0 % (0 of 35) | |
Methyl | 46.3 % (38 of 82) | 75.6 % (31 of 41) | 17.1 % (7 of 41) |
1. transmembrane domain of human nicastrin
MAHHHHHHAS KELELITLTV GFGILIFSLI VTYCINAKAD VLFIAPREPG AVSYSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | transmembrane domain of human nicastrin | [U-100% 13C; U-100% 15N] | 0.8 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | DPC | natural abundance | 200 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |