Backbone 1H, 13C, and 15N chemical shift assignments for metal-binding domain 1 of ATP7B
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.4 % (700 of 839) | 77.3 % (333 of 431) | 89.9 % (293 of 326) | 90.2 % (74 of 82) |
Backbone | 96.7 % (439 of 454) | 94.9 % (149 of 157) | 97.7 % (217 of 222) | 97.3 % (73 of 75) |
Sidechain | 72.3 % (329 of 455) | 67.2 % (184 of 274) | 82.8 % (144 of 174) | 14.3 % (1 of 7) |
Aromatic | 26.9 % (7 of 26) | 53.8 % (7 of 13) | 0.0 % (0 of 13) | |
Methyl | 85.7 % (84 of 98) | 87.8 % (43 of 49) | 83.7 % (41 of 49) |
1. entity
AGHMQVATST VRILGMTCQS CVKSIEDRIS NLKGIISMKV SLEQGSATVK YVPSVVCLQQ VCHQIGDMGF EASIAESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-13C; U-15N] | 0.5 mM | |
2 | HEPES | natural abundance | 50 mM | |
3 | DSS | natural abundance | 0.5 mM | |
4 | TCEP | natural abundance | 5 mM | |
5 | D2O | natural abundance | 10 % |