solution structure of the complex of microRNA 20b pre-element with Rbfox RRM
GSHMNTENKS QPKRLHVSNI PFRFRDPDLR QMFGQFGKIL DVEIIFNERG SKGFGFVTFE NSADADRARE KLHGTVVEGR KIEVNNATAR VMTNKKTVNP YTNG
Polymer type: polyribonucleotide polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 24.9 % (398 of 1600) | 22.7 % (193 of 849) | 30.0 % (187 of 624) | 14.2 % (18 of 127) |
Backbone | 32.7 % (284 of 869) | 37.0 % (130 of 351) | 32.5 % (136 of 418) | 18.0 % (18 of 100) |
Sidechain | 16.1 % (133 of 826) | 12.9 % (64 of 498) | 22.9 % (69 of 301) | 0.0 % (0 of 27) |
Aromatic | 39.4 % (82 of 208) | 40.4 % (42 of 104) | 44.4 % (40 of 90) | 0.0 % (0 of 14) |
Methyl | 17.4 % (16 of 92) | 15.2 % (7 of 46) | 19.6 % (9 of 46) |
1. miR-20b
GGUAGUUUUG GCAUGACUCU ACC2. Rbfox RRM
GSHMNTENKS QPKRLHVSNI PFRFRDPDLR QMFGQFGKIL DVEIIFNERG SKGFGFVTFE NSADADRARE KLHGTVVEGR KIEVNNATAR VMTNKKTVNP YTNGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | miR-20b | natural abundance | 1 mM | |
2 | sodium phosphate | natural abundance | 10 mM | |
3 | sodium chloride | natural abundance | 20 mM | |
4 | Rbfox RRM | [U-99% 13C; U-99% 15N] | 1 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | miR-20b | natural abundance | 1 mM | |
8 | sodium phosphate | natural abundance | 10 mM | |
9 | sodium chloride | natural abundance | 20 mM | |
10 | Rbfox RRM | [U-99% 13C; U-99% 15N] | 1 mM | |
11 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
12 | miR-20b | [U-99% 13C; U-99% 15N] | 1 mM | |
13 | sodium phosphate | natural abundance | 10 mM | |
14 | sodium chloride | natural abundance | 20 mM | |
15 | Rbfox RRM | natural abundance | 1 mM | |
16 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | miR-20b | [U-99% 13C; U-99% 15N] | 1 mM | |
18 | sodium phosphate | natural abundance | 10 mM | |
19 | sodium chloride | natural abundance | 20 mM | |
20 | Rbfox RRM | natural abundance | 1 mM | |
21 | H2O | natural abundance | 90 % | |
22 | D2O | natural abundance | 10 % |
Polymer type: polyribonucleotide polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 48.7 % (1557 of 3200) | 47.2 % (801 of 1698) | 50.5 % (630 of 1248) | 49.6 % (126 of 254) |
Backbone | 51.0 % (887 of 1738) | 48.0 % (337 of 702) | 52.0 % (435 of 836) | 57.5 % (115 of 200) |
Sidechain | 47.5 % (784 of 1652) | 46.7 % (465 of 996) | 51.2 % (308 of 602) | 20.4 % (11 of 54) |
Aromatic | 38.5 % (160 of 416) | 42.8 % (89 of 208) | 39.4 % (71 of 180) | 0.0 % (0 of 28) |
Methyl | 57.6 % (106 of 184) | 56.5 % (52 of 92) | 58.7 % (54 of 92) |
1. miR-20b
GGUAGUUUUG GCAUGACUCU ACC2. Rbfox RRM
GSHMNTENKS QPKRLHVSNI PFRFRDPDLR QMFGQFGKIL DVEIIFNERG SKGFGFVTFE NSADADRARE KLHGTVVEGR KIEVNNATAR VMTNKKTVNP YTNGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | miR-20b | natural abundance | 1 mM | |
2 | sodium phosphate | natural abundance | 10 mM | |
3 | sodium chloride | natural abundance | 20 mM | |
4 | Rbfox RRM | [U-99% 13C; U-99% 15N] | 1 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | miR-20b | natural abundance | 1 mM | |
8 | sodium phosphate | natural abundance | 10 mM | |
9 | sodium chloride | natural abundance | 20 mM | |
10 | Rbfox RRM | [U-99% 13C; U-99% 15N] | 1 mM | |
11 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
12 | miR-20b | [U-99% 13C; U-99% 15N] | 1 mM | |
13 | sodium phosphate | natural abundance | 10 mM | |
14 | sodium chloride | natural abundance | 20 mM | |
15 | Rbfox RRM | natural abundance | 1 mM | |
16 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | miR-20b | [U-99% 13C; U-99% 15N] | 1 mM | |
18 | sodium phosphate | natural abundance | 10 mM | |
19 | sodium chloride | natural abundance | 20 mM | |
20 | Rbfox RRM | natural abundance | 1 mM | |
21 | H2O | natural abundance | 90 % | |
22 | D2O | natural abundance | 10 % |