BMRB: 25838

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal intrinsically disordered domain of Alb3

Authors

Sattler, M., Hennig, J.

Assembly

A3CT monomer

Entity

1. A3CT monomer (polymer, Thiol state: not present), 101 monomers, 11289.25 Da Detail

MDENASKIIS AGRAKRSIAQ PDDAGERFRQ LKEQEKRSKK NKAVAKDTVE LVEESQSESE EGSDDEEEEA REGALASSTT SKPLPEVGQR RSKRSKRKRT V

Formula weight

11289.25 Da

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.2 %, Completeness: 34.0 %, Completeness (bb): 60.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	34.0 % (389 of 1145)	22.9 % (141 of 615)	39.3 % (167 of 425)	77.1 % (81 of 105)
Backbone	60.5 % (363 of 600)	60.3 % (123 of 204)	53.4 % (159 of 298)	82.7 % (81 of 98)
Sidechain	15.9 % (102 of 641)	4.4 % (18 of 411)	37.7 % (84 of 223)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 10)	0.0 % (0 of 5)	0.0 % (0 of 5)
Methyl	25.0 % (19 of 76)	13.2 % (5 of 38)	36.8 % (14 of 38)

1. Alb3-A3CT

MDENASKIIS AGRAKRSIAQ PDDAGERFRQ LKEQEKRSKK NKAVAKDTVE LVEESQSESE EGSDDEEEEA REGALASSTT SKPLPEVGQR RSKRSKRKRT V

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Alb3-A3CT	[U-100% 13C; U-100% 15N]		0.45 mM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		150 mM
4	D2O	[U-2H]		10 %

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LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Release date

2016-01-05

Citation

Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction
Horn, A., Hennig, J., Ahmed, Y.L., Stier, G., Wild, K., Sattler, M., Hennig, J.
Nat. Commun. (2015), 6, 8875-8875, PubMed 26568381 , DOI 10.1038/ncomms9875 , Abstract

Related entities 1. A3CT monomer, : 2 : 4 entities Detail

Interaction partners 1. A3CT monomer, : 18 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail