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BMRB: 25849

2N8G

NMR Structure of the homeodomain transcription factor Gbx1[E17R,R52E} from Homo sapiens

Authors

Proudfoot, A.K., Serrano, P., Geralt, M., Wuthrich, K.

Assembly

Gbx1[E17R,R52E]

Entity

1. Gbx1[E17R,R52E] (polymer, Thiol state: all free), 71 monomers, 8297.520 Da Detail

GAPGGKSRRR RTAFTSEQLL ELRKEFHCKK YLSLTERSQI AHALKLSEVQ VKIWFQNERA KWKRIKAGNV S

Formula weight

8297.52 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 89.8 %, Completeness (bb): 97.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.8 % (795 of 885)	87.7 % (413 of 471)	91.1 % (306 of 336)	97.4 % (76 of 78)
Backbone	97.2 % (412 of 424)	99.3 % (144 of 145)	95.7 % (200 of 209)	97.1 % (68 of 70)
Sidechain	85.0 % (449 of 528)	82.5 % (269 of 326)	88.7 % (172 of 194)	100.0 % (8 of 8)
Aromatic	82.9 % (58 of 70)	82.9 % (29 of 35)	81.8 % (27 of 33)	100.0 % (2 of 2)
Methyl	97.1 % (68 of 70)	100.0 % (35 of 35)	94.3 % (33 of 35)

1. entity

GAPGGKSRRR RTAFTSEQLL ELRKEFHCKK YLSLTERSQI AHALKLSEVQ VKIWFQNERA KWKRIKAGNV S

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-98% 15N]	1.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	5 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2N8G, Strand ID: A Detail

Release date

2015-10-25

Citation

Dynamic Local Polymorphisms in the Gbx1 Homeodomain Induced by DNA Binding
Proudfoot, A.K., Geralt, M., Elsliger, M., Wilson, I.A., Wuthrich, K., Serrano, P.
Structure (2016), 24, 1372-1379, PubMed 27396829 , DOI 10.1016/j.str.2016.05.013 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 18944 released on 2016-10-19
    Title NMR Structure of the homeodomain transcription factor Gbx1 from Homo sapiens
  BMRB: 19511 released on 2016-10-19
    Title NMR Structure of the homeodomain transcription factor Gbx1 from Homo sapiens solved in the presence of the DNA sequence CGACTAATTAGTCG

Related entities 1. Gbx1[E17R,R52E], : 1 : 8 : 265 entities Detail

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC