BMRB: 25858

Solution Structure of Lacticin Q and Aureocin A53

Authors

Acedo, J.Z., van Belkum, M.J., Lohans, C.T., Towle, K.M., Miskolzie, M., Vederas, J.C.

Assembly

Lacticin Q

Entity

1. Lacticin Q (polymer, Thiol state: not present), 53 monomers, 5898.014 Da Detail

MAGFLKVVQL LAKYGSKAVQ WAWANKGKIL DWLNAGQAID WVVSKIKQIL GIK

Formula weight

5898.014 Da

Source organism

Lactococcus lactis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.6 %, Completeness (bb): 99.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	93.6 % (320 of 342)	93.6 % (320 of 342)
Backbone	99.1 % (110 of 111)	99.1 % (110 of 111)
Sidechain	90.9 % (210 of 231)	90.9 % (210 of 231)
Aromatic	100.0 % (33 of 33)	100.0 % (33 of 33)
Methyl	97.4 % (38 of 39)	97.4 % (38 of 39)

1. Lacticin Q

MAGFLKVVQL LAKYGSKAVQ WAWANKGKIL DWLNAGQAID WVVSKIKQIL GIK

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	Lacticin Q	natural abundance		0.8 mM
2	DSS	natural abundance		0.01 % w/v
3	TFE	[U-99% 2H]		25 %
4	H2O	natural abundance		75 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2N8P, Strand ID: A Detail

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Release date

2016-02-14

Citation

Nuclear Magnetic Resonance Solution Structures of Lacticin Q and Aureocin A53 Reveal a Structural Motif Conserved among Leaderless Bacteriocins with Broad-Spectrum Activity
Acedo, J.Z., van Belkum, M.J., Lohans, C.T., Towle, K.M., Miskolzie, M., Vederas, J.C.
Biochemistry (2016), 55, 733-742, PubMed 26771761 , DOI 10.1021/acs.biochem.5b01306 , Abstract

Related entities 1. Lacticin Q, : 1 : 1 : 11 entities Detail

Experiments performed 3 experiments Detail