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Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1
Authors
Ilc, G., Drmota Prebil, S., Slapsak, U., de Almeida Ribeiro, E., Pavsic, M., Zielinska, K., Hartl, M., Backman, L., Plavec, J., Lenarcic, B., Djinovic-Carugo, K.
Assembly
Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1
Entity
1. Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1 (polymer), 153 monomers, 16973.65 Da Detail

GSSISQEQMN EFRASFNHFD RDHSGTLGPE EFKACLISLG YDIGNDPQGE AEFARIMSIV DPNRLGVVTF QAFIDFMSRE TADTDTADQV MASFKILAGD KNYITMDELR RELPPDQAEY CIARMAPYTG PDSVPGALDY MSFSTALYGE SDL


Formula weight
16973.65 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing, water refinement
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.0 %, Completeness: 91.0 %, Completeness (bb): 85.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All91.0 % (1561 of 1715)97.3 % (862 of 886)81.1 % (546 of 673)98.1 % (153 of 156)
Backbone85.6 % (772 of 902)97.7 % (302 of 309)73.2 % (328 of 448)97.9 % (142 of 145)
Sidechain97.2 % (928 of 955)97.1 % (560 of 577)97.3 % (357 of 367)100.0 % (11 of 11)
Aromatic94.9 % (148 of 156)94.9 % (74 of 78)94.9 % (74 of 78)
Methyl98.6 % (138 of 140)98.6 % (69 of 70)98.6 % (69 of 70)

1. Calmodulin-Like Domain of alpha-actinin 1

GSSISQEQMN EFRASFNHFD RDHSGTLGPE EFKACLISLG YDIGNDPQGE AEFARIMSIV DPNRLGVVTF QAFIDFMSRE TADTDTADQV MASFKILAGD KNYITMDELR RELPPDQAEY CIARMAPYTG PDSVPGALDY MSFSTALYGE SDL

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6


#NameIsotope labelingTypeConcentration
1Calmodulin-Like Domain of alpha-actinin 1[U-100% 13C; U-100% 15N]1 mM
2HEPESnatural abundance20 mM
3Sodium chloridenatural abundance100 mM
4DTTnatural abundance1.5 mM
5EGTAnatural abundance0.05 mM
6H2Onatural abundance90 %
7D2Onatural abundance10 %

LACS Plot; CA
Referencing offset: -0.18 ppm, Outliers: 4 Detail
LACS Plot; CB
Referencing offset: -0.18 ppm, Outliers: 4 Detail
LACS Plot; HA
Referencing offset: 0.01 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2N8Z, Strand ID: A Detail


Release date
2016-06-26
Citation
Structure and calcium-binding studies of calmodulin-like domain of human non-muscle α-actinin-1
Drmota Prebil, S., Slapsak, U., de Almeida Ribeiro, E., Pavsic, M., Ilc, G., Zielinska, K., Hartl, M., Backman, L., Plavec, J., Lenarcic, B., Djinovic-Carugo, K.
Sci. Rep. (2016), 6, 27383-27383, PubMed 27272015 , DOI 10.1038/srep27383 ,
Related entities 1. Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1, : 1 : 1 : 6 : 74 entities Detail
Interaction partners 1. Apo form of Calmodulin-Like Domain of Human Non-Muscle alpha-actinin 1, : 90 interactors Detail
Experiments performed 14 experiments Detail
NMR combined restraints 4 contents Detail
Keywords Calcium binding protein, Calmodulin-Like Domain