Structural basis for DNA cleavage by the potent antiproliferative agent (-)-lomaiviticin A
Polymer type: polydeoxyribonucleotide
Total | 1H | |
---|---|---|
All | 100.0 % (78 of 78) | 100.0 % (78 of 78) |
Suger, PO4 | 100.0 % (56 of 56) | 100.0 % (56 of 56) |
Nucleobase | 100.0 % (22 of 22) | 100.0 % (22 of 22) |
Aromatic | 100.0 % (16 of 16) | 100.0 % (16 of 16) |
Methyl | 100.0 % (2 of 2) | 100.0 % (2 of 2) |
1. DNA (5'-D(*GP*CP*TP*AP*TP*AP*GP*CP)-3')
GCTATAGCSolvent system 100% D2O, Pressure 1 atm, Temperature 297 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA (5'-D(*GP*CP*TP*AP*TP*AP*GP*CP))-3') | natural abundance | DNA | 2.2 uM |
2 | lomaiviticin A | natural abundance | 1.1 uM | |
3 | sodium phospate | natural abundance | buffer | 10 mM |
4 | sodium chloride | natural abundance | salt | 25 mM |
5 | D2O | [U-2H] | solvent | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 297 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | DNA (5'-D(*GP*CP*TP*AP*TP*AP*GP*CP))-3') | natural abundance | DNA | 2.2 uM |
7 | lomaiviticin A | natural abundance | 1.1 uM | |
8 | sodium phospate | natural abundance | buffer | 10 mM |
9 | sodium chloride | natural abundance | salt | 25 mM |
10 | H2O | natural abundance | solvent | 90 % |
11 | D2O | [U-2H] | solvent | 10 % |