Transmembrane domain of mouse Fas/CD95 death receptor
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 28.1 % (112 of 398) | 14.7 % (31 of 211) | 35.4 % (56 of 158) | 86.2 % (25 of 29) |
Backbone | 62.7 % (104 of 166) | 47.3 % (26 of 55) | 63.1 % (53 of 84) | 92.6 % (25 of 27) |
Sidechain | 3.5 % (9 of 260) | 3.2 % (5 of 156) | 3.9 % (4 of 102) | 0.0 % (0 of 2) |
Aromatic | 0.0 % (0 of 38) | 0.0 % (0 of 19) | 0.0 % (0 of 18) | 0.0 % (0 of 1) |
Methyl | 8.0 % (4 of 50) | 8.0 % (2 of 25) | 8.0 % (2 of 25) |
1. Mouse Fas Transmembrane Domain
RNRLWLLTIL VLLIPLVFIY RKYRKRKSSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8 (±0.1), Details 15N, 13C doubled labeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Mouse Fas Transmembrane Domain | [U-100% 13C; U-100% 15N] | 1 (±0.05) mM | |
2 | DMPC | [U-100% 2H] acyl chains | 60 (±1.0) mM | |
3 | DHPC | [U-100% 2H] acyl chains | 120 (±5.0) mM | |
4 | sodium phosphate | natural abundance | 20 (±1.0) mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8 (±0.1), Details 15N, 13C, 2H triple labeled sample
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Mouse Fas Transmembrane Domain | [U-100% 13C; U-100% 15N; U-85% 2H] | 1 (±0.05) mM | |
8 | DMPC | natural abundance | 60 (±1.0) mM | |
9 | DHPC | natural abundance | 120 (±5.0) mM | |
10 | sodium phosphate | natural abundance | 20 (±1.0) mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8 (±0.1), Details Mixed isotope labeled
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | Mouse Fas Transmembrane Domain | [U-100% 15N; U-100% 2H] | 0.5 (±0.05) mM | |
14 | Mouse Fas Transmembrane Domain | [U-15% 13C] | 0.5 (±0.05) mM | |
15 | DMPC | [U-100% 2H] acyl chains | 60 (±1.0) mM | |
16 | DHPC | [U-100% 2H] acyl chains | 120 (±5.0) mM | |
17 | sodium phosphate | natural abundance | 20 (±1.0) mM | |
18 | H2O | natural abundance | 95 % | |
19 | D2O | natural abundance | 5 % |