BMRB: 25967

Solution structure of ZitP zinc finger

Authors

Campagne, S., Berge, M., Viollier, P.H., Allain, F.H-T.

Assembly

ZitP zinc finger

Entity

1. ZitP zinc finger, entity 1 (polymer), 49 monomers, 5492.229 Da Detail

MILTCPECAS RYFVDDSKVG PDGRVVRCAS CGNRWTAFKD EAELELVPR

2. ZINC ION (non-polymer), 65.409 Da

Total weight

5557.638 Da

Max. entity weight

5492.229 Da

Source organism

Caulobacter vibrioides

Exptl. method

solution NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 82.8 %, Completeness (bb): 87.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.8 % (454 of 548)	83.9 % (239 of 285)	79.1 % (170 of 215)	93.8 % (45 of 48)
Backbone	87.2 % (251 of 288)	89.8 % (88 of 98)	83.3 % (120 of 144)	93.5 % (43 of 46)
Sidechain	80.1 % (245 of 306)	80.7 % (151 of 187)	78.6 % (92 of 117)	100.0 % (2 of 2)
Aromatic	75.0 % (30 of 40)	90.0 % (18 of 20)	57.9 % (11 of 19)	100.0 % (1 of 1)
Methyl	75.0 % (36 of 48)	75.0 % (18 of 24)	75.0 % (18 of 24)

1. entity 1

MILTCPECAS RYFVDDSKVG PDGRVVRCAS CGNRWTAFKD EAELELVPR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 13C15N-labeled ZitP 1mM

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]		1 mM
2	ZINC ION	natural abundance		1 mM
3	NaPO4	natural abundance		10 mM
4	NaCl	natural abundance		50 mM
5	DTT	natural abundance		10 mM

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	direct	0.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	direct	0.0

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	direct	0.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	direct	0.0

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LACS Plot; HA

Referencing offset: 0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	direct	0.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	direct	0.0

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LACS Plot; CO

Referencing offset: 0.99 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	direct	0.0
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	direct	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2NB9, Strand ID: A Detail

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Release date

2016-02-03

Citation

Modularity and determinants of a (bi-)polarization control system from free-living and obligate intracellular bacteria
Berge, M., Campagne, S., Mignolet, J., Holden, S., Theraulaz, L., Manley, S., Allain, F.H., Viollier, P.H.
Elife (2016), 5, e20640-e20640, PubMed 28008852 , DOI 10.7554/eLife.20640 , Abstract

Related entities 1. ZitP zinc finger, entity 1, : 1 : 45 entities Detail

Experiments performed 11 experiments Detail