Solution structure of the St domain of EMCV IRES
Polymer type: polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 83.0 % (352 of 424) | 78.3 % (188 of 240) | 89.1 % (164 of 184) |
Suger, PO4 | 85.7 % (264 of 308) | 85.7 % (144 of 168) | 85.7 % (120 of 140) |
Nucleobase | 75.9 % (88 of 116) | 61.1 % (44 of 72) | 100.0 % (44 of 44) |
Aromatic | 84.6 % (88 of 104) | 73.3 % (44 of 60) | 100.0 % (44 of 44) |
1. RNA (28-MER)
GGGCUGAAGG AUGGAGACGU CUAGGCCCSolvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA (28-MER) | natural abundance | RNA | 0.5 mM |
2 | potassium phosphate | natural abundance | buffer | 10 mM |
3 | sodium chloride | natural abundance | salt | 10 mM |
4 | D2O | [U-2H] | solvent | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | RNA (28-MER) | [U-13C; U-15N]-Ade, [U-13C; U-15N]-Cyt | RNA | 0.5 mM |
11 | potassium phosphate | natural abundance | buffer | 10 mM |
12 | sodium chloride | natural abundance | salt | 10 mM |
13 | D2O | [U-2H] | solvent | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
14 | RNA (28-MER) | [U-13C; U-15N]-Gua, [U-13C; U-15N]-Ura | RNA | 0.5 mM |
15 | potassium phosphate | natural abundance | buffer | 10 mM |
16 | sodium chloride | natural abundance | salt | 10 mM |
17 | D2O | [U-2H] | solvent | 100 % |