BMRBj - BMREntryMaster

Found 1 Document (4.017 seconds)

BMRB: 26012

2NCA

Structural Model for the N-terminal Domain of Human Cdc37

Authors

Zhang, Z., Keramisanou, D., Gelis, I.

Assembly

N-terminal Domain of Human Cdc37

Entity

1. N-terminal Domain of Human Cdc37 (polymer, Thiol state: all free), 380 monomers, 44661.97 Da Detail

GHMVDYSVWD HIEVSDDEDE THPNIDTASL FRWRHQARVE RMEQFQKEKE ELDRGCRECK RKVAECQRKL KELEVAEGGK AELERLQAEA QQLRKEERSW EQKLEEMRKK EKSMPWNVDT LSKDGFSKSM VNTKPEKTEE DSEEVREQKH KTFVEKYEKQ IKHFGMLRRW DDSQKYLSDN VHLVCEETAN YLVIWCIDLE VEEKCALMEQ VAHQTIVMQF ILELAKSLKV DPRACFRQFF TKIKTADRQY MEGFNDELEA FKERVRGRAK LRIEKAMKEY EEEERKKRLG PGGLDPVEVY ESLPEELQKC FDVKDVQMLQ DAISKMDPTD AKYHMQRCID SGLWVPNSKA SEAKEGEEAG PGDPLLEAVP KTGDEKDVSV

Formula weight

44661.97 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 61.6 %, Completeness: 32.4 %, Completeness (bb): 40.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	32.4 % (1487 of 4583)	28.8 % (699 of 2431)	35.4 % (620 of 1750)	41.8 % (168 of 402)
Backbone	40.6 % (916 of 2256)	39.1 % (299 of 764)	40.3 % (453 of 1124)	44.6 % (164 of 368)
Sidechain	27.2 % (733 of 2691)	24.0 % (400 of 1667)	33.2 % (329 of 990)	11.8 % (4 of 34)
Aromatic	5.6 % (17 of 304)	8.6 % (13 of 152)	0.0 % (0 of 145)	57.1 % (4 of 7)
Methyl	86.0 % (282 of 328)	86.6 % (142 of 164)	85.4 % (140 of 164)

1. N-Cdc37

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5, Details N15 labeled sample

#	Name	Isotope labeling	Type	Concentration
1	N-Cdc37	[U-100% 15N]	protein	0.5 mM
2	NaCl	natural abundance	salt	100 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
5	N-Cdc37	U-2H; VILMA methyl 1H/13C	protein	0.5 mM
6	NaCl	natural abundance	salt	100 mM
7	D2O	[U-2H]	solvent	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
8	N-Cdc37	[U-100% 13C; U-100% 15N]	protein	0.5 mM
9	H2O	natural abundance	solvent	90 %
10	D2O	[U-2H]	solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.725 ppm	na	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	water	protons	4.725 ppm	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.725 ppm	na	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	water	protons	4.725 ppm	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.725 ppm	na	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	water	protons	4.725 ppm	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.725 ppm	na	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	water	protons	4.725 ppm	na	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2NCA, Strand ID: A Detail

Release date

2016-05-08

Citation

Molecular Mechanism of Protein Kinase Recognition and Sorting by the Hsp90 Kinome-Specific Cochaperone Cdc37
Keramisanou, D.K., Aboalroub, A.A., Zhang, Z.Z., Liu, W.L., Marshall, D.M., Diviney, A.D., Larsen, R.L., Landgraf, R.L., Gelis, I.G.
Mol. Cell (2016), 62, 260-271, PubMed 27105117 , DOI 10.1016/j.molcel.2016.04.005 , Abstract

Related entities 1. N-terminal Domain of Human Cdc37, : 14 : 14 entities Detail

Interaction partners 1. N-terminal Domain of Human Cdc37, : 258 interactors Detail

More details for 258 interactors identified by 35 citations