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BMRB: 26060

2NDG

Solution NMR structures of AF9 yeats domain in complex with histone H3 crotonylation at K18

Authors

Zeng, L., ZHOU, M.

Assembly

AF9 yeats domain in complex with histone H3 crotonylation at K18

Entity

1. AF9 yeats domain in complex with histone H3 crotonylation at K18, entity 1 (polymer, Thiol state: not reported), 141 monomers, 16577.88 Da Detail

GSHMASSCAV QVKLELGHRA QVRKKPTVEG FTHDWMVFVR GPEHSNIQHF VEKVVFHLHE SFPRPKRVCK DPPYKVEESG YAGFILPIEV YFKNKEEPRK VRFDYDLFLH LEGHPPVNHL RCEKLTFNNP TEDFRRKLLK A

2. AF9 yeats domain in complex with histone H3 crotonylation at K18, entity 2 (polymer, Thiol state: not present), 13 monomers, 1393.633 Da Detail

GGKAPRXQLA TKA

Total weight

17971.514 Da

Max. entity weight

16577.88 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

distance geometry, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 88.8 %, Completeness (bb): 90.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.8 % (1666 of 1877)	87.1 % (862 of 990)	91.2 % (671 of 736)	88.1 % (133 of 151)
Backbone	90.2 % (806 of 894)	92.1 % (279 of 303)	89.6 % (403 of 450)	87.9 % (124 of 141)
Sidechain	89.0 % (1003 of 1127)	84.9 % (583 of 687)	95.6 % (411 of 430)	90.0 % (9 of 10)
Aromatic	85.1 % (165 of 194)	83.5 % (81 of 97)	86.5 % (83 of 96)	100.0 % (1 of 1)
Methyl	100.0 % (142 of 142)	100.0 % (71 of 71)	100.0 % (71 of 71)

1. entity 1

GSHMASSCAV QVKLELGHRA QVRKKPTVEG FTHDWMVFVR GPEHSNIQHF VEKVVFHLHE SFPRPKRVCK DPPYKVEESG YAGFILPIEV YFKNKEEPRK VRFDYDLFLH LEGHPPVNHL RCEKLTFNNP TEDFRRKLLK A

2. entity 2

GGKAPRXQLA TKA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	10 mM
2	sodium chloride	natural abundance	500 mM
3	EDTA	natural abundance	2 mM
4	DTT	[U-100% 2H]	2 mM
5	H20	natural abundance	90 %
6	D2O	natural abundance	10 %
7	entity_1	13C/15N-labelled	0.5 mM
8	entity_2	13C/15N-labelled	0.25 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	sodium phosphate	natural abundance	10 mM
10	sodium chloride	natural abundance	500 mM
11	EDTA	natural abundance	2 mM
12	DTT	[U-100% 2H]	2 mM
13	D2O	natural abundance	100 %
14	entity_1	13C/15N-labelled	0.5 mM
15	entity_2	13C/15N-labelled	0.25 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2NDG, Strand ID: A, B Detail

Release date

2016-08-31

Citation

Structural Insights into Histone Crotonyl-Lysine Recognition by the AF9 YEATS Domain
Zhang, Q., Zeng, L., Zhao, C., Ju, Y., Konuma, T., ZHOU, M.
Structure (2016), 24, 1606-1612, PubMed 27545619 , DOI 10.1016/j.str.2016.05.023 , Abstract

Related entities 1. AF9 yeats domain in complex with histone H3 crotonylation at K18, entity 1, : 2 : 13 entities Detail

Related entities 2. AF9 yeats domain in complex with histone H3 crotonylation at K18, entity 2, : 44 : 6 entities Detail

Interaction partners 1. AF9 yeats domain in complex with histone H3 crotonylation at K18, entity 1, : 27 interactors Detail

More details for 27 interactors identified by 14 citations