BMRB: 26510

The Sortase A Enzyme That Attaches Proteins to the Cell Wall of Bacillus anthracis Contains an Unusual Active Site Architecture

Authors

Weiner, E.M., Robson, S., Marohn, M., Clubb, R.T.

Assembly

Ba-SrtA56

Entity

1. Ba-SrtA56 (polymer, Thiol state: all free), 210 monomers, 23151.12 Da Detail

MNKQRIYSIV AILLFVVGGV LIGKPFYDGY QAEKKQTENV QAVQKMDYEK HETEFVDASK IDQPDLAEVA NASLDKKQVI GRISIPSVSL ELPVLKSSTE KNLLSGAATV KENQVMGKGN YALAGHNMSK KGVLFSDIAS LKKGDKIYLY DNENEYEYAV TGVSEVTPDK WEVVEDHGKD EITLITCVSV KDNSKRYVVA GDLVGTKAKK

Formula weight

23151.12 Da

Source organism

Bacillus anthracis

Exptl. method

solution NMR

Data set

order_parameters

Order parameters

91 S² values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 298 K, pH 6.0 Detail

---

Pressure 1 atm, Temperature 298 K, pH 6.0

Experiment name 15N-{1H} NOE 3, 2D 1H-15N HSQC 3, 15N-{1H} NOE 2, 2D 1H-15N HSQC 1, 2D 1H-15N HSQC 2, 15N-{1H} NOE 1

List #1 order_parameter_list_1, Field strength (¹H) 600 MHz

Release date

2015-02-24

Citation

The Sortase A enzyme that attaches proteins to the cell wall of Bacillus anthracis contains an unusual active site architecture
Weiner, E.M., Robson, S., Marohn, M., Clubb, R.T.
J. Biol. Chem. (2010), 285, 23433-23443, PubMed 20489200 , DOI 10.1074/jbc.M110.135434 , Abstract

Related entities 1. Ba-SrtA56, : 2 entities Detail

Experiments performed 6 experiments Detail