BMRB: 26518

NMR solution structure of the putative transfer protein TraH from Gram-positive conjugative plasmid pIP501

Authors

Meyer, N., Fercher, C., Zangger, K., Keller, W.

Assembly

putative transfer protein TraH from Gram-positive conjugative plasmid pIP501

Entity

1. putative transfer protein TraH from Gram-positive conjugative plasmid pIP501 (polymer, Thiol state: not present), 128 monomers, 14900.24 Da Detail

SNTNQSESEK IIKEFYKTVY NYEKSQKEIS MTTVKELATD NVYQELQNEI NVNNSYSPQQ NTIQKSSVNE NEIKILAYES KDNSQQYLVT APIHQVFNGT KNDFEINQLI QIKNQKITQR TTIQLGEE

Formula weight

14900.24 Da

Source organism

Enterococcus faecalis

Exptl. method

solution NMR

Refine. method

CYANA, CNS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.5 %, Completeness (bb): 97.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.5 % (1507 of 1561)	96.2 % (791 of 822)	97.8 % (569 of 582)	93.6 % (147 of 157)
Backbone	97.4 % (744 of 764)	96.5 % (247 of 256)	98.2 % (375 of 382)	96.8 % (122 of 126)
Sidechain	96.1 % (887 of 923)	96.1 % (544 of 566)	97.5 % (318 of 326)	80.6 % (25 of 31)
Aromatic	95.6 % (86 of 90)	97.8 % (44 of 45)	93.3 % (42 of 45)
Methyl	100.0 % (132 of 132)	100.0 % (66 of 66)	100.0 % (66 of 66)

1. TRAH

SNTNQSESEK IIKEFYKTVY NYEKSQKEIS MTTVKELATD NVYQELQNEI NVNNSYSPQQ NTIQKSSVNE NEIKILAYES KDNSQQYLVT APIHQVFNGT KNDFEINQLI QIKNQKITQR TTIQLGEE

Show sample condition

Sample

Solvent system 10% D2O, 90% water, Pressure 1.000 atm, Temperature 298.000 K, pH 6.500, Details 0.5 mmol/l

#	Name	Isotope labeling	Type	Concentration
1	TRAH	[U-13C; U-15N]		0.5 mM
2	PO4	natural abundance		50 mM
3	NaCl	natural abundance		100 mM

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LACS Plot; CA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	HDO	HDO	4.773 ppm	internal	indirect	0.0
1H	HDO	HDO	4.773 ppm	internal	indirect	1.0
15N	HDO	HDO	4.771 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.14 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	HDO	HDO	4.773 ppm	internal	indirect	0.0
1H	HDO	HDO	4.773 ppm	internal	indirect	1.0
15N	HDO	HDO	4.771 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.09 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	HDO	HDO	4.773 ppm	internal	indirect	0.0
1H	HDO	HDO	4.773 ppm	internal	indirect	1.0
15N	HDO	HDO	4.771 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.32 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	HDO	HDO	4.773 ppm	internal	indirect	0.0
1H	HDO	HDO	4.773 ppm	internal	indirect	1.0
15N	HDO	HDO	4.771 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 5AIW, Strand ID: A Detail

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Release date

2016-07-12

Citation

VirB8-like protein TraH is crucial for DNA transfer in Enterococcus faecalis
Fercher, C., Probst, I., Kohler, V., Goessweiner-Mohr, N., Arends, K., Grohmann, E., Zangger, K., Meyer, N., Keller, W.
Sci. Rep. (2016), 6, 24643-24643, PubMed 27103580 , DOI 10.1038/srep24643 , Abstract

Related entities 1. putative transfer protein TraH from Gram-positive conjugative plasmid pIP501, : 1 : 1 entities Detail

Experiments performed 1 experiments Detail