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BMRB: 26530

NMR assignment of intrinsically disordered self-processing module of FrpC protein of Neisseria meningitidis

Authors

Kuban, V., Zidek, L., Novacek, J., Bumba, L.

Assembly

SPM

Entity

1. SPM (polymer, Thiol state: not present), 179 monomers, 18997.55 Da Detail

PLALDLDGDG IETVATKGFS GSLFDHNRDG IRTATGWVSA DDGLLVRDLN GNGIIDNGAE LFGDNTKLAD GSFAKHGYAA LAELDSNGDN IINAADAAFQ SLRVWQDLNQ DGISQANELR TLEELGIQSL DLAYKDVNKN LGNGNTLAQQ GSYTKTNGTT AKMGDLLLAA DNLHSRFLE

Formula weight

18997.55 Da

Source organism

Neisseria meningitidis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.0 %, Completeness: 67.0 %, Completeness (bb): 90.1 % Detail

Polymer type: polypeptide(D)

	Total	¹H	¹³C	¹⁵N
All	67.0 % (1300 of 1939)	64.1 % (635 of 991)	67.4 % (502 of 745)	80.3 % (163 of 203)
Backbone	90.1 % (966 of 1072)	88.1 % (333 of 378)	91.3 % (471 of 516)	91.0 % (162 of 178)
Sidechain	46.9 % (481 of 1025)	49.3 % (302 of 613)	46.0 % (178 of 387)	4.0 % (1 of 25)
Aromatic	0.0 % (0 of 120)	0.0 % (0 of 60)	0.0 % (0 of 58)	0.0 % (0 of 2)
Methyl	33.6 % (72 of 214)	34.6 % (37 of 107)	32.7 % (35 of 107)

1. Self-processing module of FrpC

PLALDLDGDG IETVATKGFS GSLFDHNRDG IRTATGWVSA DDGLLVRDLN GNGIIDNGAE LFGDNTKLAD GSFAKHGYAA LAELDSNGDN IINAADAAFQ SLRVWQDLNQ DGISQANELR TLEELGIQSL DLAYKDVNKN LGNGNTLAQQ GSYTKTNGTT AKMGDLLLAA DNLHSRFLE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303.2 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Self-processing module of FrpC	[U-100% 13C; U-100% 15N]	0.35 mM
2	sodium chloride	natural abundance	50 mM
3	sodium azide	natural abundance	0.1 % v/v
4	TRIS	natural abundance	5 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

Release date

2015-12-03

Citation

NMR assignment of intrinsically disordered self-processing module of the FrpC protein of Neisseria meningitidis
Kuban, V., Novacek, J., Bumba, L., Zidek, L.
Biomol. NMR Assign. (2015), 9, 435-440, PubMed 26138689 , DOI 10.1007/s12104-015-9625-z , Abstract

Related entities 1. SPM, : 6 entities Detail

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC