BMRB: 26565

Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Notch1 Transmembrane Domain Segment.

Authors

Deatherage, C.L., Lu, Z., Kim, J., Sanders, C.R.

Assembly

Notch1 transmembrane segement

Entity

1. Notch1 transmembrane segement (polymer, Thiol state: all free), 59 monomers, 6836.842 Da Detail

MGHHHHHHVQ SETVEPPPPA QLHFMYVAAA AFVLLFFVGC GVLLSRKRRR QHGQLWFPE

Formula weight

6836.842 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.1 %, Completeness: 33.8 %, Completeness (bb): 67.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	33.8 % (242 of 716)	15.1 % (56 of 371)	49.0 % (140 of 286)	78.0 % (46 of 59)
Backbone	67.4 % (232 of 344)	41.9 % (49 of 117)	79.2 % (137 of 173)	85.2 % (46 of 54)
Sidechain	12.9 % (55 of 427)	3.1 % (8 of 254)	28.0 % (47 of 168)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 102)	0.0 % (0 of 51)	0.0 % (0 of 50)	0.0 % (0 of 1)
Methyl	15.0 % (9 of 60)	6.7 % (2 of 30)	23.3 % (7 of 30)

1. Notch1

MGHHHHHHVQ SETVEPPPPA QLHFMYVAAA AFVLLFFVGC GVLLSRKRRR QHGQLWFPE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 318 K, pH 5.5, Details Sample in 15% bicelle with a q=1/3.

#	Name	Isotope labeling	Type	Concentration
1	Notch1 Transmembrane segment	[U-98% 13C; U-98% 15N]		0.5 mM
2	sodium phosphate	natural abundance		20 mM
3	imidazole	natural abundance		500 mM
4	DHPC	natural abundance		65 mM
5	DMPC	natural abundance		195 mM
6	DTT	natural abundance		2 mM
7	H2O	natural abundance		90 %
8	D2O	natural abundance		10 %

Hide sample codition

LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	4.7 ppm	internal	direct	1.0

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	4.7 ppm	internal	direct	1.0

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	water	protons	4.7 ppm	internal	direct	1.0

Hide chemical shift referencing parameters

Release date

2016-01-05

Citation

Notch Transmembrane Domain: Secondary Structure and Topology
Deatherage, C.L., Lu, Z., Kim, J., Sanders, C.R.
Biochemistry (2015), 54, 3565-3568, PubMed 26023825 , DOI 10.1021/acs.biochem.5b00456 , Abstract

Related entities 1. Notch1 transmembrane segement, : 2 : 21 entities Detail

Interaction partners 1. Notch1 transmembrane segement, : 41 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail