BMRB: 26578

NMR Assignment of Homo sapiens cytochrome c in its oxidized state

Authors

Moreno-Beltran, B., Diaz-Quintana, A.J., De la Rosa, M.A., Diaz-Moreno, I.

Assembly

cytochrome c

Entity

1. cytochrome c peptide (polymer, Thiol state: all other bound), 104 monomers, 11617.39 Da Detail

GDVEKGKKIF IMKCSQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGYSYTA ANKNKGIIWG EDTLMEYLEN PKKYIPGTKM IFVGIKKKEE RADLIAYLKK ATNE

2. HEME C (non-polymer), 618.503 Da

Total weight

12235.893 Da

Max. entity weight

11617.39 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.3 %, Completeness: 30.6 %, Completeness (bb): 61.2 % Detail

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Polymer type: polypeptide(D)

	Total	1H	15N
All	30.6 % (236 of 770)	20.2 % (134 of 662)	94.4 % (102 of 108)
Backbone	61.2 % (194 of 317)	46.1 % (100 of 217)	94.0 % (94 of 100)
Sidechain	9.3 % (42 of 453)	7.6 % (34 of 445)	100.0 % (8 of 8)
Aromatic	4.2 % (2 of 48)	2.1 % (1 of 47)	100.0 % (1 of 1)
Methyl	6.4 % (3 of 47)	6.4 % (3 of 47)

1. cytochrome c peptide

GDVEKGKKIF IMKCSQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGYSYTA ANKNKGIIWG EDTLMEYLEN PKKYIPGTKM IFVGIKKKEE RADLIAYLKK ATNE

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3, Details Buffer: 5 mM phosphate buffer pH 6.3

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 15N]		0.900 (±0.001) mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %
4	phosphate buffer	natural abundance		5 mM

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Release date

2015-05-17

Citation

Structural basis for inhibition of the histone chaperone activity of SET/TAF-Iβ by cytochrome c
Gonzalez-Arzola, K., Diaz-Moreno, I., Cano-Gonzalez, A., Diaz-Quintana, A., Velazquez-Campoy, A., Moreno-Beltran, B., Lopez-Rivas, A., De la Rosa, M.A.
Proc. Natl. Acad. Sci. U. S. A. (2015), 112, 9908-9913, PubMed 26216969 , DOI 10.1073/pnas.1508040112 , Abstract

Related entities 1. cytochrome c peptide, : 1 : 5 : 3 : 8 : 219 entities Detail

Interaction partners 1. cytochrome c peptide, : 1 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail