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Found 1 Document (0.966 seconds)

BMRB: 26580

5A3G

Structure of herpesvirus nuclear egress complex subunit M50

Authors

Leigh, K.E., Boeszoermenyi, A., Mansueto, M.S., Sharma, M., Filman, D.J., Coen, D.M., Wagner, G., Hogle, J.M., Arthanari, H.

Assembly

nuclear egress subunit M50 from murine cytomegalovirus

Entity

1. nuclear egress subunit M50 from murine cytomegalovirus (polymer, Thiol state: all free), 171 monomers, 19380.08 Da Detail

AGHMEIDKNV GADLISNTRR ILRLDENELR ITDTALICKN PNYSLCDAML TTDIVYPVEY LLSYWECRSG RTACFVFKNT GCRVSLSCYI GFPERLKDLK RVCDFNFLSV NEALVVTLAD IERIKPCDKG VLTNCVVRKS NSGMSYNIEV VAFGPDNEAE YQALLRDIYA R

Formula weight

19380.08 Da

Source organism

Murid betaherpesvirus 1

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING WITH TORSION ANGLE DYNAMICS SIMULATION FOLLOWED BY RDC AND WATERBOX REFINEMENT

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 95.9 %, Completeness: 82.3 %, Completeness (bb): 95.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.3 % (1629 of 1979)	79.1 % (809 of 1023)	84.3 % (654 of 776)	92.2 % (166 of 180)
Backbone	95.0 % (965 of 1016)	93.3 % (322 of 345)	94.9 % (479 of 505)	98.8 % (164 of 166)
Sidechain	72.8 % (820 of 1126)	71.8 % (487 of 678)	76.3 % (331 of 434)	14.3 % (2 of 14)
Aromatic	21.4 % (30 of 140)	24.3 % (17 of 70)	18.8 % (13 of 69)	0.0 % (0 of 1)
Methyl	96.2 % (204 of 212)	94.3 % (100 of 106)	98.1 % (104 of 106)

1. M50

AGHMEIDKNV GADLISNTRR ILRLDENELR ITDTALICKN PNYSLCDAML TTDIVYPVEY LLSYWECRSG RTACFVFKNT GCRVSLSCYI GFPERLKDLK RVCDFNFLSV NEALVVTLAD IERIKPCDKG VLTNCVVRKS NSGMSYNIEV VAFGPDNEAE YQALLRDIYA R

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	M50	[U-13C; U-15N]	0.23 mM
2	NaPO4	natural abundance	25 mM
3	NaCl	natural abundance	150 mM
4	DTT	natural abundance	1 mM

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
5	M50	[U-13C; U-15N]	0.23 mM
6	NaPO4	natural abundance	25 mM
7	NaCl	natural abundance	150 mM
8	DTT	natural abundance	1 mM

Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
9	M50	[U-13C; U-15N]	0.23 mM
10	NaPO4	natural abundance	25 mM
11	NaCl	natural abundance	150 mM
12	DTT	natural abundance	1 mM

Sample #4

Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
13	M50	[U-13C; U-15N]	0.23 mM
14	NaPO4	natural abundance	25 mM
15	NaCl	natural abundance	150 mM
16	DTT	natural abundance	1 mM

Chem. Shift Complete2

Sequence coverage: 43.9 %, Completeness: 52.6 %, Completeness (bb): 58.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.6 % (2081 of 3958)	50.0 % (1023 of 2046)	54.7 % (849 of 1552)	58.1 % (209 of 360)
Backbone	58.0 % (1179 of 2032)	57.4 % (396 of 690)	57.1 % (577 of 1010)	62.0 % (206 of 332)
Sidechain	48.6 % (1094 of 2252)	46.3 % (628 of 1356)	53.3 % (463 of 868)	10.7 % (3 of 28)
Aromatic	10.7 % (30 of 280)	12.1 % (17 of 140)	9.4 % (13 of 138)	0.0 % (0 of 2)
Methyl	87.3 % (370 of 424)	85.8 % (182 of 212)	88.7 % (188 of 212)

1. M50

AGHMEIDKNV GADLISNTRR ILRLDENELR ITDTALICKN PNYSLCDAML TTDIVYPVEY LLSYWECRSG RTACFVFKNT GCRVSLSCYI GFPERLKDLK RVCDFNFLSV NEALVVTLAD IERIKPCDKG VLTNCVVRKS NSGMSYNIEV VAFGPDNEAE YQALLRDIYA R

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
13	M50	[U-13C; U-15N]	0.23 mM
14	NaPO4	natural abundance	25 mM
15	NaCl	natural abundance	150 mM
16	DTT	natural abundance	1 mM

Protein Blocks Logo

Calculated from 15 models in PDB: 5A3G, Strand ID: A Detail

Release date

2015-07-12

Citation

Structure of a herpesvirus nuclear egress complex subunit reveals an interaction groove that is essential for viral replication
Leigh, K.E., Sharma, M., Mansueto, M., Boeszoermenyi, A., Filman, D.J., Hogle, J.M., Wagner, G., Coen, D.M., Arthanari, H.
Proc. Natl. Acad. Sci. U. S. A. (2015), 112, 9010-9015, PubMed 26150520 , DOI 10.1073/pnas.1511140112 , Abstract

Related entities 1. nuclear egress subunit M50 from murine cytomegalovirus, : 6 entities Detail

Experiments performed 21 experiments Detail

1 15N_TROSY

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	M50	[U-13C; U-15N]	0.23 mM
2	NaPO4	natural abundance	25 mM
3	NaCl	natural abundance	150 mM
4	DTT	natural abundance	1 mM

2 TROSY_HNCA

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	M50	[U-13C; U-15N]	0.23 mM
2	NaPO4	natural abundance	25 mM
3	NaCl	natural abundance	150 mM
4	DTT	natural abundance	1 mM

3 TROSY_HNCACB

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	M50	[U-13C; U-15N]	0.23 mM
2	NaPO4	natural abundance	25 mM
3	NaCl	natural abundance	150 mM
4	DTT	natural abundance	1 mM

4 15NNOESYhsqc

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
5	M50	[U-13C; U-15N]	0.23 mM
6	NaPO4	natural abundance	25 mM
7	NaCl	natural abundance	150 mM
8	DTT	natural abundance	1 mM

5 TROSY_HNCACO

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	M50	[U-13C; U-15N]	0.23 mM
2	NaPO4	natural abundance	25 mM
3	NaCl	natural abundance	150 mM
4	DTT	natural abundance	1 mM

6 HCCONH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
9	M50	[U-13C; U-15N]	0.23 mM
10	NaPO4	natural abundance	25 mM
11	NaCl	natural abundance	150 mM
12	DTT	natural abundance	1 mM

7 CCONH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
9	M50	[U-13C; U-15N]	0.23 mM
10	NaPO4	natural abundance	25 mM
11	NaCl	natural abundance	150 mM
12	DTT	natural abundance	1 mM

8 HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
9	M50	[U-13C; U-15N]	0.23 mM
10	NaPO4	natural abundance	25 mM
11	NaCl	natural abundance	150 mM
12	DTT	natural abundance	1 mM

9 13CHSQC

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
5	M50	[U-13C; U-15N]	0.23 mM
6	NaPO4	natural abundance	25 mM
7	NaCl	natural abundance	150 mM
8	DTT	natural abundance	1 mM

10 13NOESYhsqc

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
5	M50	[U-13C; U-15N]	0.23 mM
6	NaPO4	natural abundance	25 mM
7	NaCl	natural abundance	150 mM
8	DTT	natural abundance	1 mM

11 15N_ILV_NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
13	M50	[U-13C; U-15N]	0.23 mM
14	NaPO4	natural abundance	25 mM
15	NaCl	natural abundance	150 mM
16	DTT	natural abundance	1 mM

12 13C_ILV_NOESY

Instrument

Bruker Avance - 900 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
13	M50	[U-13C; U-15N]	0.23 mM
14	NaPO4	natural abundance	25 mM
15	NaCl	natural abundance	150 mM
16	DTT	natural abundance	1 mM

13 4D 13CHMQC-NOESY-HMQC

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
13	M50	[U-13C; U-15N]	0.23 mM
14	NaPO4	natural abundance	25 mM
15	NaCl	natural abundance	150 mM
16	DTT	natural abundance	1 mM

14 RDC_HNCO

Instrument

Varian dd2 - 600 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	M50	[U-13C; U-15N]	0.23 mM
2	NaPO4	natural abundance	25 mM
3	NaCl	natural abundance	150 mM
4	DTT	natural abundance	1 mM

15 TROSY_HNCO

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	M50	[U-13C; U-15N]	0.23 mM
2	NaPO4	natural abundance	25 mM
3	NaCl	natural abundance	150 mM
4	DTT	natural abundance	1 mM

16 TROSY_HNCOCA

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	M50	[U-13C; U-15N]	0.23 mM
2	NaPO4	natural abundance	25 mM
3	NaCl	natural abundance	150 mM
4	DTT	natural abundance	1 mM

17 13C_ILV_HSQC

Instrument

Bruker Avance - 750 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500

#	Name	Isotope labeling	Concentration
13	M50	[U-13C; U-15N]	0.23 mM
14	NaPO4	natural abundance	25 mM
15	NaCl	natural abundance	150 mM
16	DTT	natural abundance	1 mM

18 15N TROSY

Instrument

Bruker AVANCE - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500, Details M50 230 umol/l

#	Name	Isotope labeling	Concentration
17	M50	[U-13C; U-15N]	230 uM
18	NaPO4	natural abundance	25 mM
19	NaCl	natural abundance	150 mM
20	DTT	natural abundance	1 mM

19 TROSY HNCA

Instrument

Varian UNITYINOVA - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500, Details M50 230 umol/l

#	Name	Isotope labeling	Concentration
17	M50	[U-13C; U-15N]	230 uM
18	NaPO4	natural abundance	25 mM
19	NaCl	natural abundance	150 mM
20	DTT	natural abundance	1 mM

20 TROSY HNCACB

Instrument

Bruker AVANCE - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500, Details M50 230 umol/l

#	Name	Isotope labeling	Concentration
17	M50	[U-13C; U-15N]	230 uM
18	NaPO4	natural abundance	25 mM
19	NaCl	natural abundance	150 mM
20	DTT	natural abundance	1 mM

21 TROSY HNCACO

Instrument

Bruker AVANCE - 750 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 291.150 K, pH 6.500, Details M50 230 umol/l

#	Name	Isotope labeling	Concentration
17	M50	[U-13C; U-15N]	230 uM
18	NaPO4	natural abundance	25 mM
19	NaCl	natural abundance	150 mM
20	DTT	natural abundance	1 mM

nullKeywords NMR, VIRAL PROTEIN, cytomegalovirus, herpesvirus, nuclear egress

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Found 1 Document (0.966 seconds)

BMRB: 26580

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Related entities 1. nuclear egress subunit M50 from murine cytomegalovirus, : 6 entities Detail

Experiments performed 21 experiments Detail

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