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BMRB: 26593

2N56

Human Obscurin Ig59

Authors

Caldwell, T.A., Wright, N.T.

Assembly

Obscurin Ig59

Entity

1. Obscurin Ig59 (polymer, Thiol state: unknown), 104 monomers, 11248.40 Da Detail

WRLEILELLK NAAVRAGAQA RFTCTLSEAV PVGEASWYIN GAAVQPDDSD WTVTADGSHQ ALLLRSAQPH HAGEVTFACR DAVASARLTV LGLPDGLEHH HHHH

Formula weight

11248.4 Da

Source organism

Exptl. method

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.2 %, Completeness: 79.4 %, Completeness (bb): 92.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.4 % (897 of 1130)	78.0 % (446 of 572)	79.5 % (357 of 449)	86.2 % (94 of 109)
Backbone	92.5 % (570 of 616)	93.8 % (198 of 211)	91.1 % (278 of 305)	94.0 % (94 of 100)
Sidechain	68.4 % (418 of 611)	68.7 % (248 of 361)	70.5 % (170 of 241)	0.0 % (0 of 9)
Aromatic	1.0 % (1 of 100)	2.0 % (1 of 50)	0.0 % (0 of 47)	0.0 % (0 of 3)
Methyl	86.6 % (116 of 134)	85.1 % (57 of 67)	88.1 % (59 of 67)

1. Obscurin Ig59

WRLEILELLK NAAVRAGAQA RFTCTLSEAV PVGEASWYIN GAAVQPDDSD WTVTADGSHQ ALLLRSAQPH HAGEVTFACR DAVASARLTV LGLPDGLEHH HHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Obscurin Ig59	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	[U-100% 2H]		10 %
3	TRIS	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		350 uM
6	H2O	natural abundance		90 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2N56, Strand ID: A Detail

Release date

2016-07-10

Citation

Solution Structure of Ig59
Caldwell, T.A., Wright, N.T.
Not known

Related entities 1. Obscurin Ig59, : 1 : 1 : 131 entities Detail

Interaction partners 1. Obscurin Ig59, : 22 interactors Detail

More details for 22 interactors identified by 10 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Obscurin Ig59	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	[U-100% 2H]		10 %
3	TRIS	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		350 uM
6	H2O	natural abundance		90 %

2 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Obscurin Ig59	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	[U-100% 2H]		10 %
3	TRIS	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		350 uM
6	H2O	natural abundance		90 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Obscurin Ig59	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	[U-100% 2H]		10 %
3	TRIS	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		350 uM
6	H2O	natural abundance		90 %

4 3D C(CO)NH

Instrument

Bruker Avance - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Obscurin Ig59	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	[U-100% 2H]		10 %
3	TRIS	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		350 uM
6	H2O	natural abundance		90 %

5 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Obscurin Ig59	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	[U-100% 2H]		10 %
3	TRIS	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		350 uM
6	H2O	natural abundance		90 %

6 3D HNCO

Instrument

Bruker Avance - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Obscurin Ig59	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	[U-100% 2H]		10 %
3	TRIS	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		350 uM
6	H2O	natural abundance		90 %

7 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Obscurin Ig59	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	[U-100% 2H]		10 %
3	TRIS	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		350 uM
6	H2O	natural abundance		90 %

8 3D HNCACB

Instrument

Bruker Avance - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Obscurin Ig59	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	[U-100% 2H]		10 %
3	TRIS	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		350 uM
6	H2O	natural abundance		90 %

9 3D H(CCO)NH

Instrument

Bruker Avance - 600 MHz TXI probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Obscurin Ig59	[U-100% 13C; U-100% 15N]		1 mM
2	D2O	[U-100% 2H]		10 %
3	TRIS	natural abundance		20 mM
4	sodium chloride	natural abundance		50 mM
5	sodium azide	natural abundance		350 uM
6	H2O	natural abundance		90 %

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