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BMRB: 26746

5JS8

Backbone resonance assignment of an inhibitory G-alpha domain

Authors

Hagn, F., Wagner, G., Goricanec, D., Stehle, R., Egloff, P., Grigori, S., Pl ckthun, A.

Assembly

GNAI1 monomer

Entity

1. GNAI1 monomer (polymer, Thiol state: all free), 326 monomers, 37199.03 Da Detail

GASREVKLLL LGAGESGKST IVKQMKIIHE AGYSEEECKQ YKAVVYSNTI QSIIAIIRAM GRLKIDFGDS ARADDARQLF VLAGAAEEGF MTAELAGVIK RLWKDSGVQA CFNRSREYQL NDSAAYYLND LDRIAQPNYI PTQQDVLRTR VKTTGIVETH FTFKDLHFKM FDVGGQRSER KKWIHCFEGV AAIIFCVALS DYDLVLAEDE EMNRMHESMK LFDSICNNKW FTDTSIILFL NKKDLFEEKI KKSPLTICYQ EYAGSNTYEE AAAYIQCQFE DLNKRKDTKE IYTHFTCATD TKNVQFVFDA VTDVIIKNNL KDCGLF

Formula weight

37199.03 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.1 %, Completeness: 47.5 %, Completeness (bb): 78.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.5 % (1836 of 3866)	25.6 % (512 of 2003)	68.4 % (1032 of 1509)	82.5 % (292 of 354)
Backbone	78.4 % (1528 of 1950)	50.8 % (338 of 666)	93.5 % (899 of 961)	90.1 % (291 of 323)
Sidechain	26.8 % (597 of 2225)	13.0 % (174 of 1337)	49.2 % (422 of 857)	3.2 % (1 of 31)
Aromatic	13.3 % (47 of 354)	16.4 % (29 of 177)	10.3 % (18 of 174)	0.0 % (0 of 3)
Methyl	27.9 % (102 of 366)	9.3 % (17 of 183)	46.4 % (85 of 183)

1. GNAI1

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0, Details 300 M GNAI1delta31

#	Name	Isotope labeling	Concentration
1	GNAI1	[U-13C; U-15N; U-2H]	200.0 ~ 400.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	5 mM
5	MgCl2	natural abundance	5 mM
6	GMPPNP	natural abundance	5 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Release date

2016-10-03

Citation

Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding
Goricanec, D., Stehle, R., Egloff, P., Grigoriu, S., Plueckthun, A., Wagner, G., Hagn, F.
Proc. Natl. Acad. Sci. U. S. A. (2016), 113, E3629-E3638, PubMed 27298341 , DOI 10.1073/pnas.1604125113 , Abstract

Related entities 1. GNAI1 monomer, : 1 : 1 : 348 entities Detail

Interaction partners 1. GNAI1 monomer, : 37 interactors Detail

More details for 37 interactors identified by 15 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC