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BMRB: 26765

6O60

NMR backbone 1H, 13C and 15N chemical shift assignments of human S-phase kinase-associated protein 1 (Skp1)

Authors

Kachariya, N.N., Kumar, A.

Assembly

Skp1

Entity

1. Skp1 (polymer, Thiol state: all free), 168 monomers, 19069.23 Da Detail

GPLGSMPSIK LQSSDGEIFE VDVEIAKQSV TIKTMLEDLG MDDEGDDDPV PLPNVNAAIL KKVIQWCTHH KDDPPPPEDD ENKEKRTDDI PVWDQEFLKV DQGTLFELIL AANYLDIKGL LDVTCKTVAN MIKGKTPEEI RKTFNIKNDF TEEEEAQVRK ENQWCEEK

Formula weight

19069.23 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 78.5 %, Completeness (bb): 91.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.5 % (1552 of 1977)	75.1 % (780 of 1038)	81.7 % (624 of 764)	84.6 % (148 of 175)
Backbone	91.2 % (899 of 986)	90.1 % (300 of 333)	91.1 % (452 of 496)	93.6 % (147 of 157)
Sidechain	69.6 % (801 of 1151)	68.1 % (480 of 705)	74.8 % (320 of 428)	5.6 % (1 of 18)
Aromatic	0.0 % (0 of 102)	0.0 % (0 of 51)	0.0 % (0 of 48)	0.0 % (0 of 3)
Methyl	82.6 % (147 of 178)	84.3 % (75 of 89)	80.9 % (72 of 89)

1. Skp1

GPLGSMPSIK LQSSDGEIFE VDVEIAKQSV TIKTMLEDLG MDDEGDDDPV PLPNVNAAIL KKVIQWCTHH KDDPPPPEDD ENKEKRTDDI PVWDQEFLKV DQGTLFELIL AANYLDIKGL LDVTCKTVAN MIKGKTPEEI RKTFNIKNDF TEEEEAQVRK ENQWCEEK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Skp1	[U-15N]	0.7 mM
2	Skp1	[U-13C; U-15N]	1.2 mM
3	Sodium Phosphate	natural abundance	25 mM
4	NaCl	natural abundance	100 mM
5	Beta mercaptoethanol	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.24 ppm, Outliers: 1 Detail

Release date

2016-03-21

Citation

Backbone and side chain assignments of human cell cycle regulatory protein S-phase kinase-associated protein 1
Kachariya, N.N., Dantu, S.C., Kumar, A.
Biomol. NMR Assign. (2016), 10, 351-355, PubMed 27394725 , DOI 10.1007/s12104-016-9699-2 , Abstract

Related entities 1. Skp1, : 1 : 29 : 194 entities Detail

Interaction partners 1. Skp1, : 147 interactors Detail

More details for 147 interactors identified by 58 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC