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BMRB: 26775

5GJL

Backbone and side chain 1H, 13C, and 15N Chemical Shift assignments for Plasmodium falciparum SUMO.

Authors

Singh, J., Gujrati, M., Shukla, V.KUMAR., Mishra, R.KUMAR., Kumar, A.

Assembly

PfSUMO

Entity

1. PfSUMO (polymer, Thiol state: not present), 98 monomers, 10825.89 Da Detail

MGDDDSAVNN NGSSPVNNQG EHIQVKVRSP DGAEVFFKIK RKTKLEKLME VYCNRLGQSM EAVRFLYDGD RIHGDNTPEQ LGIEDGDVID AMVQQTGG

Formula weight

10825.89 Da

Source organism

Plasmodium falciparum

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 82.9 %, Completeness (bb): 97.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.9 % (913 of 1101)	80.4 % (464 of 577)	85.6 % (356 of 416)	86.1 % (93 of 108)
Backbone	97.6 % (568 of 582)	96.6 % (197 of 204)	98.2 % (278 of 283)	97.9 % (93 of 95)
Sidechain	71.1 % (431 of 606)	71.6 % (267 of 373)	74.5 % (164 of 220)	0.0 % (0 of 13)
Aromatic	0.0 % (0 of 54)	0.0 % (0 of 27)	0.0 % (0 of 27)
Methyl	86.7 % (78 of 90)	91.1 % (41 of 45)	82.2 % (37 of 45)

1. Plasmodium falciparum SUMO

MGDDDSAVNN NGSSPVNNQG EHIQVKVRSP DGAEVFFKIK RKTKLEKLME VYCNRLGQSM EAVRFLYDGD RIHGDNTPEQ LGIEDGDVID AMVQQTGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Plasmodium falciparum SUMO (PfSUMO)	[U-15N]	1 mM
2	TRIS	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	Plasmodium falciparum SUMO (PfSUMO)	[U-13C; U-15N]	1 mM
6	TRIS	natural abundance	20 mM
7	sodium chloride	natural abundance	50 mM
8	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Plasmodium falciparum SUMO (PfSUMO)	[U-13C; U-15N]	1 mM
10	TRIS	natural abundance	20 mM
11	sodium chloride	natural abundance	50 mM
12	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 2 Detail

Release date

2016-04-11

Citation

Backbone and side-chain resonance assignments of Plasmodium falciparum SUMO
Singh, J.S., Shukla, V.K., Gujrati, M., Mishra, R.K., Kumar, A.
Biomol. NMR Assign. (2017), 11, 17-20, PubMed 27699617 , DOI 10.1007/s12104-016-9712-9 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 36011 released on 2016-08-01
Title Solution structure of SUMO from Plasmodium falciparum

Related entities 1. PfSUMO, : 1 : 1 : 173 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC